LOCUS       pOME0308      1944 bp    DNA     linear   UNA 29-Jun-2016
DEFINITION  Zika NS3 with start/stop codons and kozak added TAGGED
SOURCE      Zika NS3 with start/stop codons and kozak added (Zika NS3)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak seq"
     CDS             13..1938
                     /cds_type=ORF
                     /note="Length: 1857"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             13..1866
                     /cds_type=ORF
                     /label="Zika NS3"
     Site            1867..1932
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            1933..1938
                     /site_type="mutagenized site"
                     /label="double STOP"
     Site            1939..1944
                     /site_type="mutagenized site"
                     /label="3' RE"
ORIGIN
        1 ggtaccgcca ccATGAGTGG TGCTCTATGG GATGTGCCTG CTCCCAAGGA AGTAAAAAAG
       61 GGGGAGACCA CAGATGGAGT GTACAGAGTA ATGACTCGTA GACTGCTAGG TTCAACACAA
      121 GTTGGAGTGG GAGTTATGCA AGAGGGGGTC TTTCACACTA TGTGGCACGT CACAAAAGGA
      181 TCCGCGCTGA GAAGCGGTGA AGGGAGACTT GATCCATACT GGGGAGATGT CAAGCAGGAT
      241 CTGGTGTCAT ACTGTGGTCC ATGGAAGCTA GATGCCGCCT GGGACGGGCA CAGCGAGGTG
      301 CAGCTCTTGG CCGTGCCCCC CGGAGAGAGA GCGAGGAACA TCCAGACTCT GCCCGGAATA
      361 TTTAAGACAA AGGATGGGGA CATTGGAGCG GTTGCGCTGG ATTACCCAGC AGGAACTTCA
      421 GGATCTCCAA TCCTAGACAA GTGTGGGAGA GTGATAGGAC TTTATGGCAA TGGGGTCGTG
      481 ATCAAAAATG GGAGTTATGT TAGTGCCATC ACCCAAGGGA GGAGGGAGGA AGAGACTCCT
      541 GTTGAGTGCT TCGAGCCTTC GATGCTGAAG AAGAAGCAGC TAACTGTCTT AGACTTGCAT
      601 CCTGGAGCTG GGAAAACCAG GAGAGTTCTT CCTGAAATAG TCCGTGAAGC CATAAAAACA
      661 AGACTCCGTA CTGTGATCTT AGCTCCAACC AGGGTTGTCG CTGCTGAAAT GGAGGAAGCC
      721 CTTAGAGGGC TTCCAGTGCG TTATATGACA ACAGCAGTCA ATGTCACCCA CTCTGGAACA
      781 GAAATCGTCG ACTTAATGTG CCATGCCACC TTCACTTCAC GTCTACTACA GCCAATCAGA
      841 GTCCCCAACT ATAATCTGTA TATTATGGAT GAGGCCCACT TCACAGATCC CTCAAGTATA
      901 GCAGCAAGAG GATACATTTC AACAAGGGTT GAGATGGGCG AGGCGGCTGC CATCTTCATG
      961 ACCGCCACGC CACCAGGAAC CCGTGACGCA TTTCCGGACT CCAACTCACC AATTATGGAC
     1021 ACCGAAGTGG AAGTCCCAGA GAGAGCCTGG AGCTCAGGCT TTGATTGGGT GACGGATCAT
     1081 TCTGGAAAAA CAGTTTGGTT TGTTCCAAGC GTGAGGAACG GCAATGAGAT CGCAGCTTGT
     1141 CTGACAAAGG CTGGAAAACG GGTCATACAG CTCAGCAGAA AGACTTTTGA GACAGAGTTC
     1201 CAGAAAACAA AACATCAAGA GTGGGACTTT GTCGTGACAA CTGACATTTC AGAGATGGGC
     1261 GCCAACTTTA AAGCTGACCG TGTCATAGAT TCCAGGAGAT GCCTAAAGCC GGTCATACTT
     1321 GATGGCGAGA GAGTCATTCT GGCTGGACCC ATGCCTGTCA CACATGCCAG CGCTGCCCAG
     1381 AGGAGGGGGC GCATAGGCAG GAATCCCAAC AAACCTGGAG ATGAGTATCT GTATGGAGGT
     1441 GGGTGCGCAG AGACTGACGA AGACCATGCA CACTGGCTTG AAGCAAGAAT GCTCCTTGAC
     1501 AATATTTACC TCCAAGATGG CCTCATAGCC TCGCTCTATC GACCTGAGGC CGACAAAGTA
     1561 GCAGCCATTG AGGGAGAGTT CAAGCTTAGG ACGGAGCAAA GGAAGACCTT TGTGGAACTC
     1621 ATGAAAAGAG GAGATCTTCC TGTTTGGCTG GCCTATCAGG TTGCATCTGC CGGAATAACC
     1681 TACACAGATA GAAGATGGTG CTTTGATGGC ACGACCAACA ACACCATAAT GGAAGACAGT
     1741 GTGCCGGCAG AGGTGTGGAC CAGACACGGA GAGAAAAGAG TGCTCAAACC GAGGTGGATG
     1801 GACGCCAGAG TTTGTTCAGA TCATGCGGCC CTGAAGTCAT TCAAGGAGTT TGCCGCTGGG
     1861 AAAAGAgact acaaagacca tgacggtgat tataaagatc atgatatcga ttacaaggat
     1921 gacgatgaca agtagtaact cgag
//