LOCUS       pOME0310       474 bp    DNA     linear   UNA 29-Jun-2016
DEFINITION  Zika NS4A with start/stop codons and kozak added TAGGED
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     Site            7..12
                     /site_type="mutagenized site"
                     /label=Kozak
     CDS             13..465
                     /cds_type=ORF
                     /note="Length: 453"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             13..396
                     /cds_type=ORF
                     /label=NS4A
     Site            397..462
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            463..468
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            469..474
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 ggtaccgcca ccATGGGAGC GGCTTTTGGA GTGATGGAAG CCCTGGGAAC ACTGCCAGGA
       61 CACATGACAG AGAGATTCCA GGAAGCCATT GACAACCTCG CTGTGCTCAT GCGGGCAGAG
      121 ACTGGAAGCA GGCCTTACAA AGCCGCGGCG GCCCAATTGC CGGAGACCCT AGAGACCATT
      181 ATGCTTTTGG GGTTGCTGGG AACAGTCTCG CTGGGAATCT TTTTCGTCTT GATGAGGAAC
      241 AAGGGCATAG GGAAGATGGG CTTTGGAATG GTGACTCTTG GGGCCAGCGC ATGGCTCATG
      301 TGGCTCTCGG AAATTGAGCC AGCCAGAATT GCATGTGTCC TCATTGTTGT GTTCCTATTG
      361 CTGGTGGTGC TCATACCTGA GCCAGAAAAG CAAAGAgact acaaagacca tgacggtgat
      421 tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaact cgag
//