LOCUS       pOME0322       318 bp    DNA     linear   UNA 29-Jun-2016
DEFINITION  Zika membrane glycoprotein M with start/stop codons and kozak TAGGED
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     Site            7..12
                     /site_type="mutagenized site"
                     /label=Kozak
     CDS             13..240
                     /cds_type=ORF
                     /label="membrane glycoprotein M"
     CDS             13..312
                     /cds_type=ORF
     Site            241..306
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            307..312
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            313..318
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 ggtaccgcca ccATGGCTGT GACGCTCCCC TCCCATTCCA CTAGGAAGCT GCAAACGCGG
       61 TCGCAAACCT GGTTGGAATC AAGAGAATAC ACAAAGCACT TGATTAGAGT CGAAAATTGG
      121 ATATTCAGGA ACCCTGGCTT CGCGTTAGCA GCAGCTGCCA TCGCTTGGCT TTTGGGAAGC
      181 TCAACGAGCC AAAAAGTCAT ATACTTGGTC ATGATACTGC TGATTGCCCC GGCATACAGC
      241 gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat
      301 gacaagtagt aactcgag
//