LOCUS pOME0326 1605 bp DNA linear UNA 29-Jun-2016 DEFINITION Zika envelope protein E with start/stop codons and kozak TAGGED FEATURES Location/Qualifiers Site 1..6 /site_type="mutagenized site" /label="5' RE" Site 7..12 /site_type="mutagenized site" /label="Kozak seq" CDS 13..1599 /cds_type=ORF /note="Length: 1518" /note="Found at strand: positive" /note="Start codon: ATG" CDS 13..1527 /cds_type=ORF /label="Zika envelope protein E" Site 1528..1593 /site_type="mutagenized site" /label="3X FLAG" Site 1594..1599 /site_type="mutagenized site" /label="double STOP" Site 1600..1605 /site_type="mutagenized site" /label="3' RE" ORIGIN 1 ggtaccgcca ccATGATCAG GTGCATAGGA GTCAGCAATA GGGACTTTGT GGAAGGTATG 61 TCAGGTGGGA CTTGGGTTGA TGTTGTCTTG GAACATGGAG GTTGTGTCAC CGTAATGGCA 121 CAGGACAAAC CGACTGTCGA CATAGAGCTG GTTACAACAA CAGTCAGCAA CATGGCGGAG 181 GTAAGATCCT ACTGCTATGA GGCATCAATA TCGGACATGG CTTCGGACAG CCGCTGCCCA 241 ACACAAGGTG AAGCCTACCT TGACAAGCAA TCAGACACTC AATATGTCTG CAAAAGAACG 301 TTAGTGGACA GAGGCTGGGG AAATGGATGT GGACTTTTTG GCAAAGGGAG CCTGGTGACA 361 TGCGCTAAGT TTGCATGCTC CAAGAAAATG ACCGGGAAGA GCATCCAGCC AGAGAATCTG 421 GAGTACCGGA TAATGCTGTC AGTTCATGGC TCCCAGCACA GTGGGATGAT CGTTAATGAC 481 ACAGGACATG AAACTGATGA GAATAGAGCG AAGGTTGAGA TAACGCCCAA TTCACCAAGA 541 GCCGAAGCCA CCCTGGGGGG TTTTGGAAGC CTAGGACTTG ATTGTGAACC GAGGACAGGC 601 CTTGACTTTT CAGATTTGTA TTACTTGACT ATGAATAACA AGCACTGGTT GGTTCACAAG 661 GAGTGGTTCC ACGACATTCC ATTACCTTGG CACGCTGGGG CAGACACCGG AACTCCACAC 721 TGGAACAACA AAGAAGCACT GGTAGAGTTC AAGGACGCAC ATGCCAAAAG GCAAACTGTC 781 GTGGTTCTAG GGAGTCAAGA AGGAGCAGTT CACACGGCCC TTGCTGGAGC TCTGGAGGCT 841 GAGATGGATG GTGCAAAGGG AAGGCTGTCC TCTGGCCACT TGAAATGTCG CCTGAAAATG 901 GATAAACTTA GATTGAAGGG CGTGTCATAC TCCTTGTGTA CCGCAGCGTT CACATTCACC 961 AAGATCCCGG CTGAAACACT GCACGGGACA GTCACAGTGG AGGTACAGTA CGCAGGGACA 1021 GATGGACCTT GCAAGGTTCC AGCTCAGATG GCGGTGGACA TGCAAACTCT GACCCCAGTT 1081 GGGAGGTTGA TAACCGCTAA CCCCGTAATC ACTGAAAGCA CTGAGAACTC TAAGATGATG 1141 CTGGAACTTG ATCCACCATT TGGGGACTCT TACATTGTCA TAGGAGTCGG GGAGAAGAAG 1201 ATCACCCACC ACTGGCACAG GAGTGGCAGC ACCATTGGAA AAGCATTTGA AGCCACTGTG 1261 AGAGGTGCCA AGAGAATGGC AGTCTTGGGA GACACAGCCT GGGACTTTGG ATCAGTTGGA 1321 GGCGCTCTCA ACTCATTGGG CAAGGGCATC CATCAAATTT TTGGAGCAGC TTTCAAATCA 1381 TTGTTTGGAG GAATGTCCTG GTTCTCACAA ATTCTCATTG GAACGTTGCT GATGTGGTTG 1441 GGTCTGAACA CAAAGAATGG ATCTATTTCC CTTATGTGCT TGGCCTTAGG GGGAGTGTTG 1501 ATCTTCTTAT CCACAGCTGT CTCTGCTgac tacaaagacc atgacggtga ttataaagat 1561 catgatatcg attacaagga tgacgatgac aagtagtaac tcgag //