Harsharan Singh

Harsharan Singh, MD

Professor

Office: 919-966-2421
Email: hsingh@med.unc.edu

Research Interests

Our research in the area of transplantation has culminated in the characterization and development of a novel, non-invasive, diagnostic test to diagnose a major infectious complication post kidney transplantation known as polyomavirus nephropathy. This new diagnostic technique developed by me and my colleagues at UNC, called “Haufen testing”, avoids invasive biopsy procedures, and could potentially have profound implications for the care of kidney allograft recipients worldwide. The clinical impact of this novel discovery is now being confirmed in a multi-center prospective study with unrestricted funding from Astellas Pharma, US Inc. for which we are the lead investigative center. We are the lead investigators with centers participating from the US, Canada, and Europe in developing an International Consensus Classification of Polyomavirus nephropathy. Funding for this work is currently under review with the ERA-EDTA (European Renal Association / European Dialysis and Transplantation Association).

Selected Publications

Nickeleit V, Singh HK, Kenan DJ, Mieczkowski PA. The two-faced nature of BK-polyomavirus: Lytic infection or non-lytic large-T positive carcinoma. J Pathol. 2018 Jun 21.

Nickeleit V, Singh HK, Rivier LH. Antibodies Can Extenuate Polyomavirus Infections. J Am Soc Nephrol. 2018 May 29(5):1577.

Sanchez GAM, Reinhardt A, Ramsey S, Wittkowski H, Hashkes PJ, Berkun Y, Schalm S, Murias S, Dare JA, Brown D, Stone DL, Gao L, Klausmeier T, Foell D, Jesus AA, Chapelle DC, Kim H, Dill S, Colbert R, Failla L, Kost B, O’Brien M, Reynolds JC, Folio LR, Calvo KR, Paul SM, Weir N, Brofferio A, Soldatos A, Biancotto A, Cowen EW, Digiovanna JG, Gadina M, Lipton AJ, Hadigan C, Holland SM, Fontana J, Alawad AS, Brown RJ, Rother KI, Heller T, Brooks KM, Kumar P, Brooks SR, Waldman M, Singh HK, Nickeleit V, Silk M, Prakash A, Janes JM, Ozen S, Wakim PG, Brogan PA, Macias WL, Goldbach-Mansky R. JAK1/2 inhibition with baricitinib in the treatment of autoinflammatory interferonopathies. J Clin Invest. 2018 Apr 12.

Nickeleit V, Singh HK, Randhawa P, et al. Banff Working Group on Polyomavirus Nephropathy. The Banff Working Group Classification of Definitive Polyomavirus Nephropathy: Morphologic Definitions and Clinical Correlations. J Am Soc Nephrol. 2018 Feb;29(2):680-693.

Haas M, Loupy A, Lefaucheur C, Roufosse C, Glotz D, Seron D, Nankivell BJ, Halloran PF, Colvin RB, Akalin E, Alachkar N, Bagnasco S, Bouatou Y, Becker JU, Cornell LD, van Huyen JPD, Gibson IW, Kraus ES, Mannon RB, Naesens M, Nickeleit V, Nickerson P, Segev DL, Singh HK, Stegall M, Randhawa P, Racusen L, Solez K, Mengel M. The Banff 2017 Kidney Meeting Report: Revised Diagnostic Criteria for Chronic Active T Cell-Mediated Rejection, Antibody-Mediated Rejection, and Prospects for Integrative Endpoints for Next-Generation Clinical Trials. Am J Transplant. 2018 Feb;18(2):293-307.

Gougeon F, Mikhailov A,Gibson K, Kozlowski T, Singh HK, Nickeleit V. C4d-expressing Glomerulopathy and Proteinuria post Transplantation in a Too Big for Size Mismatched Kidney Allograft: An Unusual Case with Good Outcome. Clin Nephrol. 2017 Dec;88(12):364-370.

Kenan DJ, Mieczkowski PA, Latulippe E, Côté I, Singh HK, Nickeleit V. BK Polyomavirus Genomic Integration and Large T Antigen Expression: Evolving Paradigms in Human Oncogenesis. Am J Transplant. 2017 Jun; 17(6):1674-1680.

Gasim AH, Chua JS, Wolterbeek R, Schmitz J, Weimer E, Singh HK, Nickeleit V. Glomerular C4d deposits can mark structural capillary wall remodelling in thrombotic microangiopathy and transplant glomerulopathy: C4d beyond active antibody-mediated injury: a retrospective study. Transpl Int 2017 May;30(5):519-532.

Payan Schober F, Jobson MA, Poulton CJ, Singh HK, Nickeleit V, Falk RJ, Jennette JC, Nachman PH, Pendergraft Iii WF. Clinical Features and Outcomes of a Racially Diverse Population with Fibrillary Glomerulonephritis. Am J Nephrol. 2017 Feb 4;45(3):248-256.

O’Shaughnessy MM, Hogan SL, Poulton CJ, Falk RJ, Singh HK, Nickeleit V, Jennette JC. Temporal and Demographic Trends in Glomerular Disease Epidemiology in the Southeastern United States, 1986-2015. Clin J Am Soc Nephrol. 2017 Apr 3;12(4):614-623.

Loupy A, Haas M, Solez K, Racusen L, Glotz D, Seron D, Nankivell BJ, Colvin RB, Afrouzian M, Akalin E, Alachkar N, Bagnasco S, Becker JU, Cornell L, Drachenberg C, Dragun D, De Kort H, Gibson IW, Kraus ES, Lefaucheur C, Legendre C, Liapis H, Muthukumar T, Nickeleit V, Orandi B, Park W, Rabant M, Randhawa P, Reed EF, Roufosse C, Seshan SV, Sis B, Singh HK, Schinstock C, Tambur A, Zeevi A, Mengel M.  The Banff 2015 Kidney meeting report: Current challenges in rejection classification and prospects for adopting molecular pathology.  Am J Transplant.2017 Jan;17(1):28-41.

Laskin BL, MD, Singh HK, Beier UH, Moatz T, Furth SL, Bunin N, Witte D, Goebel J, Davies SM, Dandoy C, Jodele S, Nickeleit V.  The non-invasive urinary polyomavirus Haufen test predicts BK virus nephropathy in children after hematopoietic cell transplantation: a pilot study. Transplantation, Transplantation. 2016 Oct;100(10):e81-7.

Kenan DJ, Singh HK, Nickeleit V:  The Oncogenic Potential of BK Polyomavirus is Linked to Viral Integration into the Human Genome. J Pathol. 2015 Nov;237(3):379-89

Nickeleit V and Singh HK:  Polyomaviruses and Disease: Is there more than viremia and viruria? Curr Opin Organ Transplant. 2015 Jun; 20(3):348-58.

Singh HK, Reisner H, Derebail VK, Kozlowski T, Nickeleit V:  Polyomavirus Nephropathy: Quantitative Urinary Polyomavirus-Haufen Testing Accurately Predicts the Degree of Intra-Renal Viral Disease. Transplantation. 2015 Mar; 99(3):609-15.