Hyung-Suk Kim

Hyung-Suk Kim, PhD


Office: 919-966-3539

E-mail: hyungsuk_kim@med.unc.edu

Research Interests

My current interests include homeostatic regulation in hypertension and cardiac hypertrophy. To test human system in mice, we have generated animal models by using gene targeting of gene-knockout, gene duplication, 3’UTR or 5’UTR replacements. Decreased or increased protein levels by changed gene expression are valuable phenotypes for studying development of disease states.

Selected Publications

Hathaway CK, Chang AS, Grnat R, Kim HS, Madden VJ, Bagnell CR Jr, Jennette JC, Smithies O, Kakoki M. High Elmo1 expression aggravates and low Elmo1 expression prevents diabetic nephropathy. Proc Natl Acad Sci USA. 113 (8):2218 (2016).

Hathaway CK, Grant R, Hagaman JR, Hiller S, Li F, Xu L, Change AS, Madden VJ, Bagnell CR, Rojas M, Kim HS, Wu B, Zhou B, Smithies O, Kakoki M. Endothelin-1 critically influences cardiac function via superoxide-MMP9 cascade. Proc Natl Acad Sci USA. 112 (16): 5141 (2015).

Kakoki M, Pochynyuk OM, Hathaway CM, Tomita H. Hagaman JR, Kim HS, Zaika OL, Mamenko M, Kayashima Y, Matsuki K, Hiller S, Li F, Xu L, Grant R, Bertorello AM, Smithies O. Primary aldosteronism and impaired natriuresis in mice underexpressing TGFβ1. Proc Natl Acad Sci USA. 110(14): 5600-5605 (2013).

Fox R. Kim HS, Reddick RL, Kujoth GC, Prolla TA, Tsutsumi S, Wada Y, Smithies O, Maeda N. Mitochondrial DNA polymerase editing mutation, PolgD257A, reduces the diabetic phenotype of Akita male mice by suppressing appetite. Proc Natl Acad Sci USA. 108(21): 8779-8784 (2011).

Kakoki M, Sullivan KA, Backus C, Hayes JM, Oh SS, Hua K, Gasim AM, Tomita H, Grant R, Nossov SB, Kim HS, Jennette JC, Feldman EL, Smithies O. Lack of both bradykinin B1 and B2 receptors enhances nephropathy, neuropathy, and bone mineral loss in Akita diabetic mice. Proc. Natl. Acad. Sci. USA 107(22): 10190-10195 (2010).

Takahashi N, Li F, Hua K, Deng J, Wang CH, Bowers RR, Bartness TJ, Kim HS, Harp JB. Increased energy expenditure, dietary fat wasting, and resistance to diet-induced obesity in mice lacking Renin. Cell Metab. 6: 506-512 (2007).

Lee, G., Makhanova, N., Caron, K., Lopez, M.L.S., Gomez, R.A., Smithies, O. and Kim, H.S. Homeostatic responses in the adrenal cortex to the absence of aldosterone in mice. Endocrinol.146: 2650-2656 (2005).

Kakoki, M., Tsai, Y-S., Kim, H.S., Hatada, S., Ciavatta, D.J., Takahashi, N., Arnold, L.W., Maeda, N. and Smithies, O. Altering the expression in mice of genes by modifying their 3’ regions. Developmental Cell, 6: 597-606 (2004).

Kim, H.S., Lee, G., John, S.W.M., Maeda, N. and Smithies, O. Molecular phenotyping for analyzing subtle genetic effects in mice: Application to an angiotensinogen gene titration. Proc. Natl. Acad. Sci.USA, 99: 4602-4607(2002).

Kim, H.S., Maeda, N., Oh, G.T., Fernandez, L.G., Gomez, R.A. and Smithies, O. Homeostasis in mice with genetically decreased angiotensinogen is primarily by an increased number of renin-producing cells. J. Biol. Chem. 274: 14210-14217 (1999).

Kim, H.S., Krege, J.H., Kluckman, K.D., Hagaman, J.R., Hodgin, J.B., Best, C.F., Jennett, J.C., Coffman, T.M., Maeda, N. and Smithies, O. Genetic control of blood pressure and the angiotensinogen locus. Proc. Natl. Acad. Sci. USA 92: 2735-2739 (1995).

Smithies, O. and Kim, H.S. Targeted gene duplication and disruption for analyzing quantitative genetic traits in mice. Proc. Natl. Acad. Sci. USA 91: 3612-3615 (1994).

Kim, H.S. and Smithies, O. Recombinant fragment assay for gene targeting based on the polymerase chain reaction. Nucleic Acids Res. 16: 8887-8903 (1988).