{"id":2252,"date":"2014-07-11T19:30:00","date_gmt":"2014-07-11T23:30:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/pharm\/calabreselab\/calabreselab-2\/protocols\/"},"modified":"2018-07-18T10:34:02","modified_gmt":"2018-07-18T14:34:02","slug":"protocols","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/pharm\/calabreselab\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"
\n

Genomics<\/h3>\n

Tufts NextGen sequencing primer<\/a> (pdf)<\/p>\n

Annealing Truseq adapters<\/a> (doc)<\/p>\n

Truseq adapter sequences<\/a> (xlsx)<\/p>\n

Kapa mRNA-seq library preparation<\/a> (pdf)<\/p>\n

NEB Next ChIP-Seq library preparation<\/a> (doc)<\/p>\n

Stat Quest<\/a> (website)<\/p>\n

Cloning<\/span><\/h3>\n

Alkaline lysis mini preps<\/a> (docx)<\/p>\n

Glycerol Stocks<\/a> (docx)<\/p>\n

Gibson Master Mix <\/a>(docx)<\/p>\n

Gibson Assembly<\/a> (docx)<\/p>\n

RNA<\/h3>\n

Gel extraction of RNA<\/a> (docx)<\/p>\n

Northern Blots<\/a> (doc)<\/p>\n

Nuclear Extraction<\/a> (docx)<\/p>\n

Pyrosequencing<\/a> (docx)<\/p>\n

Gentle extraction of RNA from chromatin-enriched fraction<\/a> (docx)<\/p>\n

DNA<\/h3>\n

Southern Blotting<\/a> (doc)<\/p>\n

Imaging<\/h3>\n

Fluorescent labeling of DNA probes for FISH<\/a> (doc)<\/p>\n

Immunofluorescence<\/a> (docx)<\/p>\n

RNA FISH\/IF<\/a> (docx)<\/p>\n

IF\/DNA\/RNA FISH<\/a> (docx)<\/p>\n

Cell culture<\/h3>\n

Trophoblast stem cells<\/a> (docx)<\/p>\n

XEN cells<\/a> (docx)<\/p>\n

ESCs<\/a> (doc)<\/p>\n

epiSCs<\/a> (docx)<\/p>\n

293T transfection<\/a> (doc)<\/p>\n

Optimization of electroporation with Neon<\/a> (doc)<\/p>\n

Electroporation with Neon<\/a> (doc)<\/p>\n

Cellular Fractionation<\/a> (doc)<\/p>\n

TETRIS assay<\/a> (docx)<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Genomics Tufts NextGen sequencing primer (pdf) Annealing Truseq adapters (doc) Truseq adapter sequences (xlsx) Kapa mRNA-seq library preparation (pdf) NEB Next ChIP-Seq library preparation (doc) Stat Quest (website) Cloning Alkaline lysis mini preps (docx) Glycerol Stocks (docx) Gibson Master Mix (docx) Gibson Assembly (docx) RNA Gel extraction of RNA (docx) Northern Blots (doc) Nuclear Extraction … Read more<\/a><\/p>\n","protected":false},"author":32335,"featured_media":0,"parent":0,"menu_order":15,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2252","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"\nProtocols - Calabrese Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/pharm\/calabreselab\/protocols\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protocols - 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