The NextSeq500 was purchased by the Department in August of 2014. It is available for use by all members of the Department.<\/p>\n
For general info on the Illumina platform and the NextSeq, check out the short informational videos on their website<\/a>. There are 3 videos worth watching (even if you don\u2019t plan to use TruSeq kits): Sequencing:Illumina Technology, NextSeq500 Overview, TruSeq:RNA Sample Prep<\/p>\n If you would like to submit samples for sequencing, please look through the information outlined below. Users are responsible for preparing their own samples and purchasing the appropriate reagent kit for the NextSeq.<\/p>\n Purchase a kit through Illumina off of UNC\u2019s standing quote, or use another provider, like Kapa (standing quote), or NEB or Agilent. If you are purchasing your own adapters<\/a>, follow the guidelines listed here<\/a> to prepare them. If you are curious to learn how adapters work, read this<\/a>.<\/p>\n Once you have prepared your libraries, quantify the DNA using a fluorometer or nanodrop, and estimate the average size of the library via agarose gel electrophoresis. Then, pool your libraries to a final concentration of 15nM in pH 8\/8.5 10mM Tris buffer and submit to Brian for sequencing.<\/p>\nPreparing Samples and Quality Control<\/h3>\n