{"id":2968,"date":"2024-08-02T11:29:51","date_gmt":"2024-08-02T15:29:51","guid":{"rendered":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/?page_id=2968"},"modified":"2024-09-17T16:34:36","modified_gmt":"2024-09-17T20:34:36","slug":"guidelines-and-protocols","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/resources\/guidelines-and-protocols\/","title":{"rendered":"Guidelines &#038; Protocols"},"content":{"rendered":"<p>Please refer to the <a href=\"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/sample-submission\/\">sample submission page<\/a> for instructions on how to submit samples to the MAP Core.<\/p>\n<p>Project-specific guidelines are available upon request.<\/p>\n<h2 class=\"www-somHeader\">Avoiding Keratin Contamination<\/h2>\nKeratin contamination is a common problem with mass spectrometry analysis. Sources of keratin contamination include contact with skin, hair, dust, clothing, and latex gloves. If keratin is present in high concentrations, signal loss and a decrease in protein identifications may occur. Below are a few tips that will help minimize keratin contamination during sample preparation.<\/p>\n<ul>\n<li>Avoid storing gels in plastic wrap or similar material. Instead, use newand cleaned plastic or glass gel trays.<\/li>\n<li>Refrain from letting anything come into contact with the gel, such as dust or hair.<\/li>\n<li>Perform all sample preparation steps in a laminar flow hood, if possible.<\/li>\n<li>Wipe down surfaces with water and ethanol\/methanol.<\/li>\n<li>ALWAYS USE NON-LATEX GLOVES and wear lab coats.<\/li>\n<li>Store consumables and reagents in covered containers, such as gels, liquid and powdered reagents, pipette tips, etc.<\/li>\n<li>No wool clothing in the lab.<\/li>\n<li>To avoid BSA or other contaminates from western blot assays: do not use the same plates or gel containers.<\/li>\n<li>For gel electrophoresis, wash all glass plates thoroughly with 70% ethanol prior to casting an SDS-PAGE gel. After completing gel electrophoresis step, disassemble the glass plates in a laminar flow hood. Destain the gel in a clean container that has been rinsed thoroughly with 70% ethanol or methanol\/acetonitrile.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Please refer to the sample submission page for instructions on how to submit samples to the MAP Core. Project-specific guidelines are available upon request. Keratin contamination is a common problem with mass spectrometry analysis. Sources of keratin contamination include contact with skin, hair, dust, clothing, and latex gloves. If keratin is present in high concentrations, &hellip; <a href=\"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/resources\/guidelines-and-protocols\/\" aria-label=\"Read more about Guidelines &#038; Protocols\">Read more<\/a><\/p>\n","protected":false},"author":112631,"featured_media":0,"parent":2273,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2968","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/2968","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/users\/112631"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/comments?post=2968"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/2968\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/2273"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/media?parent=2968"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}