This service provides broad profiling of polar and semi-polar metabolites using hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution mass spectrometry on a Thermo Orbitrap Exploris 240. Data are acquired in both positive and negative ionization modes to maximize metabolome coverage. Peak areas are reported for both known metabolites and unknown features, with compound annotation supported by spectral library matching.<\/p>\n
This service provides quantitative profiling of 18 metabolites involved in glycolysis and the tricarboxylic acid (TCA) cycle, two central pathways in cellular energy metabolism. Samples are analyzed using high-resolution liquid chromatography\u2013mass spectrometry (LC-MS) to enable sensitive, reproducible detection.<\/p>\n
We offer custom targeted metabolomics panels tailored to your research needs, using both LC-MS and capillary electrophoresis\u2013mass spectrometry (CE-MS) platforms. Panels can be designed to focus on specific pathways, compound classes, or research questions, with flexible assay development and consultation available.<\/p>\n
Please email Whitney Stutts<\/a> and Crystal Pace<\/a> for more information on these and other assays coming soon.<\/p>\n","protected":false},"excerpt":{"rendered":" This service provides broad profiling of polar and semi-polar metabolites using hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution mass spectrometry on a Thermo Orbitrap Exploris 240. Data are acquired in both positive and negative ionization modes to maximize metabolome coverage. Peak areas are reported for both known metabolites and unknown features, with compound annotation … Read more<\/a><\/p>\n","protected":false},"author":112631,"featured_media":0,"parent":2258,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3008","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/3008","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/users\/112631"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/comments?post=3008"}],"version-history":[{"count":8,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/3008\/revisions"}],"predecessor-version":[{"id":3373,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/3008\/revisions\/3373"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/pages\/2258"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/proteomics-metabolomics\/wp-json\/wp\/v2\/media?parent=3008"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}