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Molecular and mechanistic toxicology, primary and organotypic in vitro models, live cell imaging/fluorescent biosensors, redox dysfunction/oxidative stress, inhalation toxicology, translational research, in vitro to in vivo extrapolation, environmental and public health epigenetics

Institution: U.S. Environmental Protection Agency


Voice: (919) 843-8031


Dr. McCullough’s lab takes a translational research approach that incorporates primary cell and organotypic in vitro models with clinical research (controlled human exposures) to study the role of cellular and molecular mechanisms in mediating the local and systemic effects of exposure to inhaled chemicals.  His laboratory utilizes primary cell/organotypic in vitro models, live cell imaging of fluorescent biosensors, and both traditional and advanced molecular biology/biochemistry methods to characterize the relationship between redox dysfunction/oxidative stress, inflammation, cell signaling pathway activation, epigenetic changes, gene expression, and cell-specific functional outcomes.  In addition to identifying the mechanisms involved in the effects of toxic exposures, Dr. McCullough’s research also aims to identify biomarkers of toxic exposure effects, predicting susceptible populations, and identifying factors that can be used to mitigate adverse exposure outcomes.