{"id":2220,"date":"2014-05-23T15:15:00","date_gmt":"2014-05-23T19:15:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/vectorecore\/aav-custom-prep\/"},"modified":"2026-06-11T13:46:42","modified_gmt":"2026-06-11T17:46:42","slug":"aav-custom-prep","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vectorcore\/aav-custom-prep\/","title":{"rendered":"AAV Custom Vectors and Other Services"},"content":{"rendered":"
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Custom AAV Vectors<\/h2>\n

Custom AAV vector production at the UNC Vector Core is designed to support projects ranging from pilot studies to\u00a0large-scale\u00a0research needs. We offer flexible production options using a scalable,\u00a0high<\/span>\u2011purity\u00a0suspension<\/span>\u2011based\u00a0platform.<\/span>\u00a0<\/span><\/p>\n

Custom Standard Size Vectors: <\/span>\u00a0<\/span><\/h3>\n

The Vector Core provides custom AAV vector production in serotypes AAV1\u20136, 8, 9, Olig001, rAAV2Retro, and DJ<\/strong>. Please\u00a0inquire about\u00a0additional\u00a0serotypes. Vectors are produced using a\u00a0triple\u2011transfection protocol in suspension of HEK293 cells and purified by column chromatography. This serum-free, antibiotic\u2011free\u00a0system yields\u00a0higher\u2011purity\u00a0vectors with fewer empty particles and is scalable to Wave bioreactors.\u00a0<\/span><\/p>\n

For standard-size custom preparations, a minimum of 300 \u00b5g of plasmid DNA is required (plasmid amplification services are available for an additional fee). Typical turnaround time is approximately 4\u20135 weeks. We guarantee a minimum total titer of 1 \u00d7 10\u00b9\u00b9 viral genomes, with average yields of ~1\u20134 \u00d7 10\u00b9\u00b2 vg\/mL in a final volume of 1\u20131.3\u202fmL; yield is construct<\/span>\u2011dependent. Standard custom AAV production includes titer determination by qPCR and silver stain analysis.<\/span>\u00a0<\/span><\/p>\n

For quotes, contact Vector Core<\/a>. <\/span>\u00a0<\/span><\/p>\n

Large Scale Custom AAV Production:<\/span>\u00a0<\/span><\/h3>\n

The Vector Core offers large-scale, research-grade custom AAV vector production in serotypes AAV1\u20136, 8, 9, Olig001, rAAV2Retro, and DJ. Please inquire about additional serotypes. Production is available at 1\u202fL, 2\u202fL, 5\u202fL, 10\u202fL, and 20\u202fL culture volumes, with 2\u202fL and larger preparations produced in Wave bags. Vectors are generated using a triple<\/span>\u2011transfection\u00a0protocol in\u00a0suspension of\u00a0HEK293 cells and purified by column chromatography. This\u00a0serum<\/span>\u2011free,\u00a0antibiotic<\/span>\u2011free\u00a0system yields\u00a0higher<\/span>\u2011purity\u00a0vectors with fewer empty particles and is scalable to\u00a0large<\/span>\u2011volume\u00a0Wave bioreactors. Final yield is\u00a0construct<\/span>\u2011dependent, with a typical 10\u202fL preparation yielding approximately 5 \u00d7 10\u00b9\u00b3 to 2 \u00d7 10\u00b9\u2074 viral genomes. Minimum plasmid DNA requirements are \u2265500\u202f\u00b5g for 1\u202fL, \u2265900\u202f\u00b5g for 2\u202fL, and \u22652\u202fmg for 5\u202fL productions; plasmid amplification services are available if needed (submit 1\u201310\u202f\u00b5g). Cost savings are available for larger Wave cultures of the same construct; please\u00a0contact Vector Core<\/a> for production volumes larger than 20\u202fL<\/span>.<\/span><\/p>\n

Other Services:<\/h2>\n

The UNC Vector Core offers a range of ancillary services to support vector production and downstream research needs. These services include plasmid amplification, reporter gene transfections, and other custom options designed to complement standard and custom AAV workflows.<\/span>\u00a0<\/span><\/p>\n

Amplification Services<\/span><\/b>\u00a0<\/span><\/h3>\n

If you are interested in custom vector production but do not have sufficient plasmid DNA, we provide high-quality plasmid amplification service. Our amplified plasmids are suitable for a wide range of applications, including transfection and AAV vector production.\u00a0<\/span>\u00a0<\/span><\/p>\n

We specialize in amplifying customer-provided plasmids under optimized conditions to ensure consistency, purity, and reliability. If you would like to use this service, please send us 1\u201310 \u00b5g of your plasmid DNA.<\/span>\u00a0<\/span><\/p>\n

Available plasmid DNA yield options:<\/span><\/b>\u00a0<\/span><\/p>\n