{"id":2277,"date":"2014-07-17T19:55:00","date_gmt":"2014-07-17T23:55:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/vectorecore\/ordering-faq\/other-information\/"},"modified":"2025-09-18T11:47:15","modified_gmt":"2025-09-18T15:47:15","slug":"other-information","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vectorcore\/ordering-faq\/other-information\/","title":{"rendered":"Other Information"},"content":{"rendered":"
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How do I request AAV plasmids?<\/h2>\n

Our AAV packaging and helper plasmids are banked at the NGVB (National Gene Vector Biorepository). To request AAV plasmids via MTA or to view plasmid maps and sequences, please visit the NGVB website<\/a>.<\/strong><\/p>\n

To request plasmids from a collaborating lab (Deisseroth, Boyden etc), please contact that lab directly.<\/p>\n

What do I do if I don\u2019t see expression?<\/h2>\n

Please email our lab manager, Genlin Hu<\/strong><\/a>\u00a0with your lot number and description of the issue. If you\u2019re working with a collaborator construct and we don\u2019t have any other reported issues on the lot, we will refer you to the source lab for further information and clarification.<\/p>\n

If the issue is due to our production or purification of the virus, then we will replace at no cost. If it is determined the experimental design, animal model, serotype\/construct choice, method of administration, etc. is the cause, then we cannot replace the virus at no cost.<\/p>\n

Please see notes on the safety page<\/a> regarding AAV handling and storage. If virus isn\u2019t kept at -80 until ready to use, undergoes multiple freeze\/thaws or is aliquoted smaller than 10ul and\/or aliquoted into tubes prone to protein binding, that can result in loss of virus and low or no expression. <\/strong><\/em><\/p>\n

What testing do you provide?<\/h2>\n