Welcome to the Vironomics Core!

Purpose

The Vironomics core is designed to facilitate research at UNC by performing large-scale Real Time PCR reactions and next generation sequencing using Roche 454 GS Junior. A Tecan Freedom Evo© is used to set up reactions in a 384-well plate, which is then directly run by the synchronised Roche Lightcycler 480 II©. This is a fast and effective way to amplify samples with RT-PCR. Because the Freedom Evo is directly integrated with the Lightcycler, after manually adding 96-well plates containing samples or primers to the Freedom Evo, the plates can be run 24 hours/day. The GS Junior System brings the power of 454 Sequencing technology directly to the research laboratory bench top. The same proven long-read sequencing performance as the Genome Sequencer FLX System is scaled to suit the needs of individual labs. Quickly proceed from DNA to data to discovery with a simple sample preparation workflow, overnight sequencing and data processing, and a dedicated suite of data analysis software.

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