{"id":121,"date":"2017-08-07T15:24:19","date_gmt":"2017-08-07T19:24:19","guid":{"rendered":"https:\/\/med.sites.unc.edu\/vironomics\/sequencing-1\/our-best-ion-runs\/"},"modified":"2017-11-30T12:05:09","modified_gmt":"2017-11-30T17:05:09","slug":"our-best-ion-runs","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/sequencing-1\/our-best-ion-runs\/","title":{"rendered":"Examples of Our Seq Runs"},"content":{"rendered":"

<\/p>\n

<\/p>\n

<\/p>\n

Here we will keep examples of our Ion Torrent sequencing runs and metrics, click for larger images<\/p>\n

Examples of our runs on Ion Torrent S5<\/strong><\/h2>\n

(Click for larger image)<\/p>\n

\"June
~200bp S5 run 102 million reads!
(poly(A) RNAseq) 540 chip
<\/strong><\/p>\n

\"19,034,414
~400bp S5 run 19 million reads!
530 chip
<\/strong><\/p>\n

\"Best ~600bp S5 run 11 million reads!
530 chip
<\/strong><\/span><\/p>\n

\n\n

PHRED\/Q Scores<\/strong><\/span><\/h2>\n

PHRED(33)\/Q Scores are a measure of accuracy at each base position. It isn’t enough to say we sequenced 6.5 million reads (1 sample) from a 600bp fragment protocol if by the 600th base-pair the sequence is unreliable; luckily our Ion Torrent S5 sequencing is producing great base-pair qualities all the way to the end.<\/span><\/p>\n

Figures produced on Qiagen CLC Bio Genomics Workbench software by Jay Zhang<\/em>
<\/span><\/p>\n

\"Vironomics<\/span><\/p>\n

\"Vironomics<\/span><\/p>\n

LEGEND<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n
PHRED(33)\/Q Score<\/strong><\/span><\/th>\nIncorrect<\/strong><\/span><\/th>\nAccuracy<\/strong><\/span><\/th>\n<\/tr>\n
10<\/span><\/td>\n1 in 10<\/span><\/td>\n90%<\/span><\/td>\n<\/tr>\n
20<\/span><\/td>\n1 in 100<\/span><\/td>\n99%<\/span><\/td>\n<\/tr>\n
30<\/span><\/td>\n1 in 1000<\/span><\/td>\n99.9%<\/span><\/td>\n<\/tr>\n
40<\/span><\/td>\n1 in 10,000<\/span><\/td>\n99.99%<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


<\/strong><\/span><\/p>\n


<\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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