{"id":121,"date":"2017-08-07T15:24:19","date_gmt":"2017-08-07T19:24:19","guid":{"rendered":"https:\/\/med.sites.unc.edu\/vironomics\/sequencing-1\/our-best-ion-runs\/"},"modified":"2017-11-30T12:05:09","modified_gmt":"2017-11-30T17:05:09","slug":"our-best-ion-runs","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/sequencing-1\/our-best-ion-runs\/","title":{"rendered":"Examples of Our Seq Runs"},"content":{"rendered":"

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Here we will keep examples of our Ion Torrent sequencing runs and metrics, click for larger images<\/p>\n

Examples of our runs on Ion Torrent S5<\/strong><\/h2>\n

(Click for larger image)<\/p>\n

\"June
~200bp S5 run 102 million reads!
(poly(A) RNAseq) 540 chip
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\"19,034,414
~400bp S5 run 19 million reads!
530 chip
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\"Best ~600bp S5 run 11 million reads!
530 chip
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\n\n

PHRED\/Q Scores<\/strong><\/span><\/h2>\n

PHRED(33)\/Q Scores are a measure of accuracy at each base position. It isn’t enough to say we sequenced 6.5 million reads (1 sample) from a 600bp fragment protocol if by the 600th base-pair the sequence is unreliable; luckily our Ion Torrent S5 sequencing is producing great base-pair qualities all the way to the end.<\/span><\/p>\n

Figures produced on Qiagen CLC Bio Genomics Workbench software by Jay Zhang<\/em>
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\"Vironomics<\/span><\/p>\n

\"Vironomics<\/span><\/p>\n

LEGEND<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n
PHRED(33)\/Q Score<\/strong><\/span><\/th>\nIncorrect<\/strong><\/span><\/th>\nAccuracy<\/strong><\/span><\/th>\n<\/tr>\n
10<\/span><\/td>\n1 in 10<\/span><\/td>\n90%<\/span><\/td>\n<\/tr>\n
20<\/span><\/td>\n1 in 100<\/span><\/td>\n99%<\/span><\/td>\n<\/tr>\n
30<\/span><\/td>\n1 in 1000<\/span><\/td>\n99.9%<\/span><\/td>\n<\/tr>\n
40<\/span><\/td>\n1 in 10,000<\/span><\/td>\n99.99%<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n


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<\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Here we will keep examples of our Ion Torrent sequencing runs and metrics, click for larger images Examples of our runs on Ion Torrent S5 (Click for larger image) ~200bp S5 run 102 million reads!(poly(A) RNAseq) 540 chip ~400bp S5 run 19 million reads!530 chip ~600bp S5 run 11 million reads!530 chip PHRED\/Q Scores PHRED(33)\/Q … Read more<\/a><\/p>\n","protected":false},"author":33839,"featured_media":0,"parent":37,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-121","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/121","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/users\/33839"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/comments?post=121"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/121\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/37"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/media?parent=121"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}