{"id":123,"date":"2017-03-23T19:30:00","date_gmt":"2017-03-23T23:30:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/vironomics\/sequencing-1\/str-cell-line-authentication\/"},"modified":"2017-12-20T08:35:54","modified_gmt":"2017-12-20T13:35:54","slug":"str-cell-line-authentication","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/sequencing-1\/str-cell-line-authentication\/","title":{"rendered":"STR Cell Line Authentication"},"content":{"rendered":"

<\/p>\n

<\/p>\n

<\/p>\n

For NIH Grant Application authentication of key biological and\/or chemical resources<\/p>\n

ALL WORK DONE IN-HOUSE<\/strong><\/span><\/h2>\n

Ion Torrent Precision ID GlobalFiler\u2122 NGS STR Panel<\/a> of forensically relevant Short Tandem Repeats (STRs) from genomic DNA.<\/h2>\n


<\/b><\/h3>\n

Service provided:<\/b> DNA extraction<\/span> from cell pellet + sequenced with the GlobalFiler STR panel 33 markers<\/span> + comparative analysis with the DSMZ<\/a>* database<\/span> (ATCC, DSMZ, JCRB, and RIKEN) in reference to other cell lines using the following loci: D5S818, D13S317, D7S820, D16S539, VWA, TH01, AM, TPOX, and CSF1PO.<\/h3>\n<\/p>\n

Cost:<\/b> $234<\/b> internal (2x Viral Load clinical rate, until rates are re-done and approved)<\/h4>\n

Note: In order to bring the cost down for you, samples will be run in batches of 12. If you submit 12 cell pellets these will immediately enter the queue. Any sample submission<\/a> >12 will be batched with others and may take 3 months. <\/i><\/p>\n


<\/i><\/p>\n

The panel targets 33 markers<\/b>:<\/h5>\n

\u2022 20 autosomal STR CODIS and Expanded CODIS loci<\/p>\n

\u2022 1 Y-chromosome STR locus<\/p>\n

\u2022 1 autosomal Non-CODIS 02 locus<\/p>\n

\u2022 3 autosomal Low Probability of Identity (PI) (0.09) STR loci<\/p>\n

\u2022 5 autosomal Next Generation Sequencing (NGS) STR loci<\/p>\n

\u2022 1 indel polymorphic marker on the Y chromosome (Y indel)<\/p>\n

\u2022 X and Y amelogenin, the sex determining marker <\/p>\n

SUBMISSION<\/strong><\/p>\n

    \n
  1. See this page<\/a> to set-up billing first<\/li>\n
  2. Prepare cell pellet and freeze: prefer 1×10^6 cells\/pellet washed in PBS<\/span><\/li>\n
  3. Fill out Sample Submission Form<\/a><\/span><\/li>\n
      \n
    1. List full name of cell line (column B\/D)<\/span><\/li>\n
    2. List number of cells in the pellet (column C)
      <\/span><\/li>\n
    3. List passage number (if known) (column D)<\/span><\/li>\n
    4. “Volume sent”-not applicable (column E)<\/span><\/li>\n<\/ol>\n
    5. Submit frozen samples and paperwork (see steps #4 and 5<\/a>)
      <\/span><\/li>\n<\/ol>\n

      <\/h3>\n

      *DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), English translation: German Collection of Microorganisms and Cell Cultures GmbH<\/em><\/p>\n

      \n","protected":false},"excerpt":{"rendered":"

      For NIH Grant Application authentication of key biological and\/or chemical resources ALL WORK DONE IN-HOUSE Ion Torrent Precision ID GlobalFiler\u2122 NGS STR Panel of forensically relevant Short Tandem Repeats (STRs) from genomic DNA. Service provided: DNA extraction from cell pellet + sequenced with the GlobalFiler STR panel 33 markers + comparative analysis with the DSMZ* … Read more<\/a><\/p>\n","protected":false},"author":33839,"featured_media":0,"parent":37,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-123","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/123","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/users\/33839"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/comments?post=123"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/123\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/37"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/media?parent=123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}