{"id":124,"date":"2016-01-07T20:55:00","date_gmt":"2016-01-08T01:55:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/vironomics\/sequencing-1\/unofficial-guide-to-sequencing\/"},"modified":"2018-01-30T07:39:30","modified_gmt":"2018-01-30T12:39:30","slug":"unofficial-guide-to-sequencing","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/sequencing-1\/unofficial-guide-to-sequencing\/","title":{"rendered":"Unofficial guide to sequencing"},"content":{"rendered":"

<\/p>\n

<\/p>\n

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Next-generation sequencing technologies are being updated constantly so sometimes it is hard to choose the technology that is best for your research. Below we’ve collected an unofficial table to help compare the instruments that were current at the beginning of 2016.<\/p>\n

*Note: We assembled this list from experience, vendor sites, other cores, and word-of-mouth. Please let us know if you find any errors.<\/p>\n

Yes<\/span><\/strong>Yes<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/th>\nIllumina<\/strong><\/th>\nIonTorrent<\/strong><\/th>\nPacBio<\/strong><\/th>\n<\/tr>\n
Instrument<\/td>\nMiSeq<\/strong><\/td>\nNextSeq<\/strong><\/td>\nHiSeq<\/strong><\/td>\nPGM<\/strong><\/span><\/td>\nS5<\/strong><\/span><\/td>\nProton<\/strong><\/td>\nRS II<\/strong><\/td>\nSequel System*<\/strong><\/td>\n<\/tr>\n
Technology Strength<\/span><\/td>\nSequencing depth<\/span><\/td>\nCombination<\/span><\/td>\nRead length<\/span><\/td>\n<\/tr>\n
Available at V. Core ?<\/span><\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n\u00a0YES<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Total Workflow Time<\/span><\/td>\n~2-5 days<\/span><\/td>\n~3 days<\/span><\/td>\n~8 days<\/span><\/td>\n~3 days<\/span><\/td>\n~3-4 days<\/span><\/td>\n~2 days<\/span><\/td>\n~1 day<\/span><\/td>\n<\/td>\n<\/tr>\n
Typical Price per library prep<\/span><\/td>\n$172<\/span><\/td>\n>$147<\/span><\/td>\n>$155<\/span><\/td>\n$206<\/span><\/td>\n$206<\/span><\/td>\n$172<\/span><\/td>\n>$309<\/span><\/td>\n<\/td>\n<\/tr>\n
Typical Price per run<\/span><\/td>\n$823 – $1,750<\/span><\/td>\n>$1,143<\/span><\/td>\n$913 – $2,449<\/span><\/td>\n$1,339<\/span><\/td>\n$1,339-1,715<\/span><\/td>\n$1,199<\/span><\/td>\n$699<\/span><\/td>\n<\/td>\n<\/tr>\n
Read length of output (bp)<\/span><\/td>\n300<\/span><\/td>\n75-300<\/span><\/td>\n36 – 250<\/span><\/td>\n200-400<\/span><\/td>\n200-600<\/span><\/td>\n200<\/span><\/td>\n>20,000<\/span><\/td>\n>20,000<\/span><\/td>\n<\/tr>\n
# of reads per run<\/span><\/td>\n25M<\/span><\/td>\n130-400M<\/span><\/td>\n300M – 4B<\/span><\/td>\n4 – 5.5M<\/span><\/td>\n15 – 80M<\/span><\/td>\n60 – 80M<\/span><\/td>\n0.055M<\/span><\/td>\n0.385M<\/span><\/td>\n<\/tr>\n
Sequencing time (h)<\/span><\/td>\n~56<\/span><\/td>\n~11-29<\/span><\/td>\n~144<\/span><\/td>\n7<\/span><\/td>\n7 – 22<\/span><\/td>\n2 – 4<\/span><\/td>\n0.5 – 4<\/span><\/td>\n0.5 – 6<\/span><\/td>\n<\/tr>\n
Data size of output (GB)<\/span><\/td>\n15<\/span><\/td>\n16-120<\/span><\/td>\n9 – 1000<\/span><\/td>\n1.2 – 2<\/span><\/td>\n6 – 15<\/span><\/td>\n10<\/span><\/td>\n0.5 – 1<\/span><\/td>\n3.5 – 7<\/span><\/td>\n<\/tr>\n
Uses amplification of template ?<\/span><\/td>\nYes<\/span><\/td>\nYes<\/span><\/td>\nYes<\/span><\/td>\nYes<\/span><\/td>\nYes<\/span><\/td>\nYes<\/span><\/td>\nNo<\/span><\/td>\nNo<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

*introduced Sep 2015<\/b><\/p>\n

References:<\/b><\/p>\n

Miseq: http:\/\/www.illumina.com\/systems\/miseq.html<\/a> , http:\/\/www.illumina.com\/systems\/miseq\/performance_specifications.html<\/a><\/span>
\nNextSeq: http:\/\/www.illumina.com\/systems\/nextseq-sequencer.html<\/a>
\n<\/span>Hiseq2000: http:\/\/www.illumina.com\/Documents\/systems\/hiseq\/datasheet_hiseq_systems.pdf<\/a><\/span><\/p>\n

PGM: https:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/PGM-Specification-Sheet.pdf<\/a>
\nS5: https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/next-generation-sequencing\/ion-torrent-next-generation-sequencing-workflow\/ion-torrent-next-generation-sequencing-run-sequence\/ion-s5-ngs-targeted-sequencing\/ion-s5-specifications.html<\/a>
\nProton: https:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/CO06326_Proton_Spec_Sheet_FHR.pdf<\/a><\/span><\/p>\n

RS II: http:\/\/www.pacb.com\/wp-content\/uploads\/2015\/09\/PacBio_RS_II_Brochure.pdf<\/a>
\nSequel: http:\/\/www.pacb.com\/wp-content\/uploads\/2015\/09\/Revolutionize-Genomics-with-SMRT-Sequencing.pdf<\/a><\/span><\/p>\n

Others\u2019 comparison of different sequencing technologies:<\/b><\/p>\n

http:\/\/www.biomedcentral.com\/1471-2164\/15\/96<\/span><\/a>
\nhttp:\/\/www.nature.com\/news\/next-generation-genome-sequencers-compared-1.10497<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Next-generation sequencing technologies are being updated constantly so sometimes it is hard to choose the technology that is best for your research. Below we’ve collected an unofficial table to help compare the instruments that were current at the beginning of 2016. *Note: We assembled this list from experience, vendor sites, other cores, and word-of-mouth. Please … Read more<\/a><\/p>\n","protected":false},"author":33839,"featured_media":0,"parent":37,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-124","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/124","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/users\/33839"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/comments?post=124"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/124\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/37"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/media?parent=124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}