{"id":128,"date":"2013-06-20T14:35:00","date_gmt":"2013-06-20T18:35:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/vironomics\/services\/real-time-pcr-arrays-available\/"},"modified":"2019-10-16T13:53:49","modified_gmt":"2019-10-16T17:53:49","slug":"real-time-pcr-arrays-available","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/services\/real-time-pcr-arrays-available\/","title":{"rendered":"Real-time PCR (qPCR) arrays available"},"content":{"rendered":"

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We offer various qPCR arrays that include anywhere from 5 to 768 primer-pairs per assay. Our assays include: KSHV, EBV, HCMV, HSV-1, HSV-2, RRV, Viral Load, microRNA, NFkappaB, and P53response.<\/p>\n


\n<\/span>The Vironomics Core ran 19,968<\/span> qPCR reactions during one month! <\/strong><\/div>\n

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For each validated assay we have: standard operating procedures, quality controls built in, and trained personnel. Please see below for a few publications that have resulted from each assay.<\/b><\/p>\n


\n<\/b>SAMPLE SUBMISSION<\/strong><\/p>\n

qPCR requests should be submitted to Dr. Dirk Dittmer and\/or the Vironomics Core Manager, email is best. Once approved, Viral Array samples should be prepared following the SOP of qPCR mRNA Preparation<\/a>. <\/strong>Overall, 100uL of cDNA is required for submission. Viral Load samples should be submitted as 1mL of primary sample.<\/p>\n

ASSAYS AVAILABLE<\/strong><\/p>\n

Our individual viral arrays<\/span> (RT-qPCR) include the following viruses: KSHV, EBV, HSV-1, HSV-2, RRV, and HCMV. We also offer a Viral Load assay (qPCR) that includes KSHV, EBV, CMV, and HSV-1 all in one assay.<\/p>\n