{"id":39,"date":"2010-09-02T20:45:00","date_gmt":"2010-09-03T00:45:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/vironomics\/about-us-2\/equipment\/"},"modified":"2026-02-15T07:56:01","modified_gmt":"2026-02-15T12:56:01","slug":"equipment","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/about-us-2\/equipment\/","title":{"rendered":"Equipment"},"content":{"rendered":"<h4>Long-Read Sequencers:<\/h4>\n<ul>\n<li>\u00a0PacBio Seq II long read sequencer: 6,000 &#8211; 20,000 bp length.<\/li>\n<li>Abi Sanger Sequencer: 500 -800 bp Sanger (gold-standard) sequencer for orthogonal validation of SNVs.<\/li>\n<\/ul>\n<h4>Short-Read Sequencers:<\/h4>\n<ul>\n<li>Ion Torrent S5: 200-400bp read-length Next-Gen Sequencer<\/li>\n<\/ul>\n<h4>Robots:<\/h4>\n<ul>\n<li>Tecan Evo 50: Robot with 96 well pipetting head for post-PCR clean-up<\/li>\n<li>Tecan Evo 150: Robot with integrated 384-well Roche LC480 real-time PCR reactions<\/li>\n<li>CAS-1200 (Corbett Res.): Robot pipetting robot which pipettes 96\/384 well PCR reactions with 0.1 microL accuracy (aka <strong>QIAgility<\/strong>)<\/li>\n<\/ul>\n<h4>Supporting Equipment:<\/h4>\n<ul>\n<li><strong>Ion Chef<\/strong> for automated AmpliSeq library to chip loading for Ion Torrent sequencers<\/li>\n<li>Roche LightCycler 480 (384-well and 96-well)<\/li>\n<li>Centrifuge for 96 and 384 well plate centrifugation<\/li>\n<li>Pippin Prep automated gel fragments sizing and elution station<\/li>\n<li>Agilent Bioanalyzer 2100<\/li>\n<li>Caliper GX 384 well analyzer<\/li>\n<li>Tecan M200 fluorometer with NanoDrop capability<\/li>\n<li>Qubit 3.0 DNA analyzer (Life Technologies)<\/li>\n<li>Digital PCR machine (ABI\/Life Technologies)<\/li>\n<\/ul>\n<h4>Servers and Computers:<\/h4>\n<ul>\n<li>Penguin Computing Tempest 4400 computing server<\/li>\n<li>FTP server for data delivery<\/li>\n<li>Multiple workstations, including bioinformatics software, for analysis<\/li>\n<\/ul>\n<h4>Example usage<\/h4>\n<div class=\"pageBreak\">This video published by the Journal of Visualized Experiments shows how samples may be set up and aliquoted by the Tecan Freedom Evo<sup>\u00ae<\/sup>, as well as quantified by the Roche Lightcycler<sup>\u00ae<\/sup> 480 II.<\/div>\n<div class=\"pageBreak\"><\/div>\n<p class=\"aux\" style=\"line-height: 1.334em;margin: 0em 0pt 0em 0pt\"><em>Chugh P, Tamburro K, Dittmer DP (2010). Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays. J Vis Exp. 46. <\/em><\/p>\n<p class=\"aux\" style=\"line-height: 1.334em;margin: 0em 0pt 0em 0pt\"><span class=\"smallFont\"><a class=\"external-link\" title=\"\" href=\"http:\/\/www.jove.com\/index\/details.stp?id=2210, doi: 10.3791\/2210\" target=\"_self\" rel=\"noopener noreferrer\">http:\/\/www.jove.com\/index\/details.stp?id=2210, doi: 10.3791\/2210<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Long-Read Sequencers: \u00a0PacBio Seq II long read sequencer: 6,000 &#8211; 20,000 bp length. Abi Sanger Sequencer: 500 -800 bp Sanger (gold-standard) sequencer for orthogonal validation of SNVs. Short-Read Sequencers: Ion Torrent S5: 200-400bp read-length Next-Gen Sequencer Robots: Tecan Evo 50: Robot with 96 well pipetting head for post-PCR clean-up Tecan Evo 150: Robot with integrated &hellip; <a href=\"https:\/\/www.med.unc.edu\/vironomics\/about-us-2\/equipment\/\" aria-label=\"Read more about Equipment\">Read more<\/a><\/p>\n","protected":false},"author":76119,"featured_media":0,"parent":2,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-39","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/39","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/users\/76119"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/comments?post=39"}],"version-history":[{"count":2,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/39\/revisions"}],"predecessor-version":[{"id":713,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/39\/revisions\/713"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/2"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/media?parent=39"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}