{"id":616,"date":"2018-07-02T09:01:50","date_gmt":"2018-07-02T13:01:50","guid":{"rendered":"https:\/\/www.med.unc.edu\/vironomics\/?page_id=616"},"modified":"2026-02-15T08:18:48","modified_gmt":"2026-02-15T13:18:48","slug":"agilent-tapestation","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/vironomics\/services\/agilent-tapestation\/","title":{"rendered":"Agilent TapeStation"},"content":{"rendered":"<blockquote><p>This service is included in all sequencing projects conducted at the UNC Vironomics core and contained in the library preparation rate. It is no longer available by itself!<\/p>\n<p><strong>Sample submission: <\/strong>Please specify which option you want below, and at submission if any special post-analysis (zoom to sample, specific size regions)<\/p><\/blockquote>\n<p><span style=\"text-decoration: underline\">High Sensitivity (HS) D1000 DNA<\/span>:<\/p>\n<ul>\n<li>Double-stranded DNA<\/li>\n<li>assess 35-1000bp<\/li>\n<li>Concentration-Minimum 5pg\/\u00b5L. <strong>Dilute to below 1000pg\/\u00b5L (assess by NanoDrop)<\/strong>.<\/li>\n<li>5\u00b5L if &lt;15 samples, in tubes\n<ul>\n<li>OR 2\u00b5L if &gt;15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><span style=\"text-decoration: underline\">High Sensitivity (HS) D5000 DNA<\/span>:<\/p>\n<ul>\n<li>Double-stranded DNA<\/li>\n<li>assess 100-5000bp<\/li>\n<li>Concentration-Minimum 5pg\/\u00b5L.<strong> Dilute to below 1000pg\/\u00b5L (assess by NanoDrop).<\/strong><\/li>\n<li>5\u00b5L if &lt;15 samples, in tubes\n<ul>\n<li>OR 2\u00b5L if &gt;15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><span style=\"text-decoration: underline\">D1000 DNA<\/span>:<\/p>\n<ul>\n<li>Double-stranded DNA<\/li>\n<li>assess 35-1000bp<\/li>\n<li>Concentration-Minimum 0.1ng\/\u00b5L. <strong>Dilute to below 50ng\/\u00b5L (assess by NanoDrop).<\/strong><\/li>\n<li>5\u00b5L if &lt;15 samples, in tubes\n<ul>\n<li>OR 1\u00b5L if &gt;15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><span style=\"text-decoration: underline\">RNA<\/span>:<\/p>\n<ul>\n<li>Total RNA. <strong>Please specify if prokaryotic or eukaryotic<\/strong> (viral ~eukaryotic)<\/li>\n<li>Concentration-Minimum 5ng\/\u00b5L. <strong>Dilute to below 500ng\/\u00b5L (assess by NanoDrop)<\/strong>.<\/li>\n<li>3\u00b5L if &lt;15 samples, in tubes\n<ul>\n<li>OR 1\u00b5L if &gt;15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"list-style-type: none\"><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>This service is included in all sequencing projects conducted at the UNC Vironomics core and contained in the library preparation rate. It is no longer available by itself! Sample submission: Please specify which option you want below, and at submission if any special post-analysis (zoom to sample, specific size regions) High Sensitivity (HS) D1000 DNA: &hellip; <a href=\"https:\/\/www.med.unc.edu\/vironomics\/services\/agilent-tapestation\/\" aria-label=\"Read more about Agilent TapeStation\">Read more<\/a><\/p>\n","protected":false},"author":33839,"featured_media":0,"parent":38,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-616","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/616","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/users\/33839"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/comments?post=616"}],"version-history":[{"count":1,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/616\/revisions"}],"predecessor-version":[{"id":730,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/616\/revisions\/730"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/pages\/38"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/vironomics\/wp-json\/wp\/v2\/media?parent=616"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}