{"id":204,"date":"2014-09-19T22:10:49","date_gmt":"2014-09-19T22:10:49","guid":{"rendered":"http:\/\/zebrafish.web.unc.edu\/?page_id=204"},"modified":"2024-10-17T16:11:52","modified_gmt":"2024-10-17T20:11:52","slug":"transgenic-generation","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/zebrafish\/gene-editing\/transgenic-generation\/","title":{"rendered":"Transgenic Generation"},"content":{"rendered":"

To generate a transgenic line:<\/h2>\n
    \n
  1. Prepare tools\n
      \n
    1. Identify the promoter for the gene of interest using standard molecular biology techniques<\/li>\n
    2. Clone promoter upstream of a reporter
      \n
      \"Whole
      Whole zebrafish kdrl-gfp courtesy of Kevin Mouillesseaux <\/em><\/figcaption><\/figure>
      \"\"
      The GATA:dsRed construct makes hemopoetic precursors (precursors to blood cells) glow red. Courtesy of Josh Blair, Bautch Lab<\/figcaption><\/figure>\n

      (fluorescent protein or other reporter) using Tol2 kit<\/p>\n<\/li>\n<\/ol>\n<\/li>\n

    3. Make mosaic fish using Tol2 transposase kit<\/li>\n
    4. Evaluate expression pattern in F1 fish<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

      To generate a transgenic line: Prepare tools Identify the promoter for the gene of interest using standard molecular biology techniques Clone promoter upstream of a reporter (fluorescent protein or other reporter) using Tol2 kit Make mosaic fish using Tol2 transposase kit Evaluate expression pattern in F1 fish<\/p>\n","protected":false},"author":81071,"featured_media":0,"parent":2320,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"layout":"","cellInformation":"","apiCallInformation":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-204","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"\nTransgenic Generation - Zebrafish Aquaculture Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/zebrafish\/gene-editing\/transgenic-generation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Transgenic Generation - 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