Deisseroth

In-Stock AAV Vectors – Dr. Karl Deisseroth, 100ul Aliquots

We are pleased to stock optogenetic constructs from Dr. Karl Deisseroth at Stanford University. For first time orders of these viruses, Laboratory PIs please contact Dr. Karl Deisseroth (deissero@stanford.edu) and Charu Ramakrishnan (charur@stanford.edu) for an MTA.Please upload a copy of your MTA when placing your online order.

The following constructs are available in AAV2 and AAV5 with the following exceptions: GFAP constructs available in AAV1, 2, 5 & 8, AAV-EF1a-mCherry-IRES-WGA-Cre available in AAV2, 5 & 8, C1V1(t/t) and SwiChRCA in AAV2, 5 and DJ, 20ul concentrated high titer AAV-hSyn-hChR2(H134R)-EYFP (≥1x10E13 vg/ml) available in AAV6 and Opto-XRs in AAV5. For other serotypes, please place an order for a custom prep.

In Stock AAV Price per 100ul

• External Academic: $220
• UNC Investigator: $150
• Corporate: $340

Optical Excitation: ChR2

• AAV-CaMKIIa-hChR2(H134R)-EYFP
• AAV-CaMKIIa-hChR2(H134R)-mCherry
• AAV-hSyn-hChR2(H134R)-EYFP
• AAV-hSyn-hChR2(H134R)-mCherry
• 20ul – High Titer AAV-hSyn-hChR2(H134R)-EYFP (AAV6 only)

Cre-inducible AAV: DIO-AAV

• AAV-EF1a-DIO-hChR2(H134R)-EYFP
• AAV-EF1a-DIO-hChR2(H134R)-mCherry

Third-generation Optogenetic Inhibition: eNpHR3.0

• AAV-EF1a-DIO-eNpHR3.0-EYFP
• AAV-EF1a-DIO-eNpHR3.0-mCherry
• AAV-CaMKIIa-eNpHR3.0-EYFP
• AAV-CaMKIIa-eNpHR3.0-mCherry
• AAV-hSyn-eNpHR3.0-EYFP
• AAV-hSyn-eNpHR3.0-mCherry
• AAV-hThy1-eNpHR3.0-EYFP

Red-shifted Optical Excitation: C1V1 Variants

• AAV-CaMKIIa-C1V1(E122T/E162T)-TS-EYFP
• AAV-CaMKIIa-C1V1(E122T/E162T)-TS-mCherry
• AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-EYFP
• AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-mCherry
• AAV-CaMKIIa-C1V1(E162T)-p2A-EYFP

C1V1(t/t) (AAV2, 5, DJ)

• AAV-hSyn-C1V1(t/t)-TS-EYFP
• AAV-hSyn-C1V1(t/t)-TS-p2A-EYFP

Stabilized Step Function Opsins: SSFOs

• AAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
• AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
• AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFP
• AAV-EF1a-DIO-hChR2(C128S/D156A)-mCherry

Second-generation Ultrafast Optogenetic Control

• AAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
• AAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
• AAV-CaMKII-hChR2(E123A)-EYFP
• AAV-CaMKII-hChR2(E123A)-mCherry
• AAV-EF1a-DIO-hChR2(E123T/T159C)-EYFP
• AAV-EF1a-DIO-hChR2(E123T/T159C)-mCherry
• AAV-EF1a-DIO-ChR2(T159C)-EYFP
• AAV-EF1a-DIO-ChR2(T159C)-mCherry
• AAV-EF1a-DIO-ChR2(E123A)-EYFP
• AAV-EF1a-DIO-ChR2(E123A)-mCherry
• AAV-hSyn-ChR2(E123A)-EYFP

Third-generation Optogenetic Inhibition: Arch 3.0 & ArchT 3.0

• AAV-CaMKIIa-eArch3.0-EYFP
• AAV-hSyn-eArch3.0-EYFP
• AAV-EF1a-DIO-eArch3.0-EYFP
• AAV-CaMKIIa-eArchT3.0-EYFP

Transsynaptic Tracers: WGA-Cre (AAV2, 5 & 8)

• AAV-EF1a-mCherry-IRES-WGA-Cre

Ultrafast Optogenetic Control: ChETA

• AAV-EF1a-DIO-ChETA-EYFP

Cell Filling Variants

• AAV-CaMKII-hChR2(T159C)-p2A-EYFP-WPRE
• AAV-CaMKII-hChR2(T159C)-p2A-mCherry-WPRE
• AAV-CaMKII-hChR2(E123T/T159C)-p2A-EYFP-WPRE
• AAV-CaMKII-hChR2(E123T/T159C)-p2A-mCherry-WPRE
• AAV-CaMKIIa-C1V1(E162T)-TS-p2A-EYFP-WPRE
• AAV-CaMKIIa-C1V1(E162T)-TS-p2A-mCherry-WPRE
• AAV-CaMKIIa-C1V1(t/t)-TS-p2A-EYFP-WPRE
• AAV-CaMKIIa-C1V1(t/t)-TS-p2A-mCherry-WPRE
• AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-EYFP-WPRE
• AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-mCherry-WPRE
• AAV-EF1a-DIO-C1V1(E162T)-TS-p2A-EYFP-WPRE
• AAV-EF1a-DIO-C1V1(E162T)-TS-p2A-mCherry-WPRE
• AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-EYFP-WPRE
• AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-mCherry-WPRE

SwiChRCA (AAV2, 5, DJ)

• AAV-CaMKIIa-SwiChRCA-TS-EYFP
• AAV-EF1a-DIO-SwiChRCA-TS-EYFP-WPRE
• AAV-hSyn-SwiChRCA-TS-EYFP

Control Fluorophores

• AAV-CaMKIIa-EYFP
• AAV-CaMKIIa-mCherry
• AAV-EF1a-DIO-EYFP
• AAV-EF1a-DIO-mCherry
• AAV-hSyn-EYFP
• AAV-hSyn-mCherry

Optical Excitation: ChR2-GFAP (AAV1, 2, 5 & 8)

• AAV-GFAP-hChR2(H134R)-EYFP
• AAV-GFAP-hChR2(H134R)-mCherry