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High Throughput Genomic Sequencing Facility

The High Throughput Sequencing Facility (HTSF) offers comprehensive library services, NextGen sequencing and alternative technologies including long reads. In addition to generating high quality genomic and transcriptomic data, we also provide technological support to users to ensure maximum data value. Our goal is to help researchers make biological sense out of their data. This may require us to develop and implement novel applications for current platforms. We aim to provide high quality data for cutting edge technologies that are an excellent value for the service. We are part of the Integrated Genomics Cores.
Update on COVID-19 : HTSF Updates University Updates

Integrating Expertise, Enabling Data Driven Discovery, Informing Patient Care

Announcements

WELCOME TO THE HTSF SUBMISSION SYSTEM

We are open for all materials’ submission as Library Prep group and Sequencing group continue processing. If you already have an account at the HTSF, submit as needed. The Customer Service group will approve submission and work with you for drop off of the samples. Due to SOM COVID rules, we are scheduling 30m windows for drop off to insure social distancing for the lab and studies.

We continue to meet with studies for consults via zoom. Please reach out to customer service team with a brief description of your needs and we will help arrange a consult. If you have submission batch status questions, please reach out to the customer service staff.

We look forward to working with you.

Thank you

The HTSF Team

Director’s Welcome

Corbin Jones

On behalf of the Office of the Vice Chancellor for Research, the School of Medicine, and the University of North Carolina; welcome to the High Throughput Sequencing Facility (HTSF) web site. The HTSF is now part of the Integrated Genomics Cores (IGC) which also includes Mammalian Genotyping Core (MGC) and the Functional Genomics Microarray Core. Just prior to the COVID shut down, HTSF brought on a new director of the IGC: Greg Bowen.
We are pleased to have the opportunity to collaborate with you and support your research needs. The team is committed to providing the highest quality genomics and sequencing solutions, tailored to provide you the data you need to succeed, as cost effectively as possible. The HTSF have a long history in supporting research and scientific breakthroughs, and in helping understand a wide range of diseases and pathogens. We work with numerous vendors as beta testing sites for new services. Our R&D group can apply existing techniques in novel manners or help trouble shoot assays for you.
– Corbin Jones

Greg Bowen

I am still learning but I am thrilled to be a member of this extraordinary group of scientists and to play a role in supporting so many vital research projects, both here at UNC and with our many external customers and collaborators. This new HTSF website is one of many ongoing initiatives to continuously improve the services and the quality we provide. With an eye on the future and a focus on the present, we continue to offer both tried and true and new, next generation sequencing technology solutions. I look forward to working with you and hope the IGC’s and HTSF can become you long term partner and collaborator!
– Greg Bowen

What’s New

  • NovaSeq 6000 vs HighSeq 2500, Evaluation by mRNA-seq

    This is a presentation on the comparison of the same series of libraries run on both the HS2500 and the Novaseq. These were TruSeq stranded mRNA. Pools of 4 libraries were run on the HiSeq 2500/ paired end/ 50x. The same pools were then pooled into larger pools (pools of pools) to be run on … Continued

  • Scientific Highlight – Mammary Tumor Models

    By creating new mammary tumor models, we find that tumor mutation burden and specific immune cells are associated with response. The core generated both single cell and bulk RNA-seq gene expression data to help this team of Lineberger researchers identify mechanisms mediating responses to immune checkpoint inhibitors using mouse models of triple-negative breast cancer. Hollern … Continued