As new NGS platforms become available, studies are required to shift the sequencings plans. This is a comparison of the same series of libraries run on older HiSeq systems (HS2500 and HS4000) and the newer Nextseq and Novaseq platforms. These were TruSeq stranded mRNA. Pools of 4 libraries were run on the HiSeq/ paired end/ … Read more

HTSF supported Clinical Proteomic Tumor Analysis Consortium by providing microRNA sequencing.

Scientific Highlights _ Allogeneic Hematopoietic Stem Cell Transplantation

This is a presentation on the comparison of the same series of libraries run on both the HS2500 and the Novaseq. These were TruSeq stranded mRNA. Pools of 4 libraries were run on the HiSeq 2500/ paired end/ 50x. The same pools were then pooled into larger pools (pools of pools) to be run on … Read more

By creating new mammary tumor models, we find that tumor mutation burden and specific immune cells are associated with response. The core generated both single cell and bulk RNA-seq gene expression data to help this team of Lineberger researchers identify mechanisms mediating responses to immune checkpoint inhibitors using mouse models of triple-negative breast cancer. Hollern … Read more