Lab Publications
For a comprehensive and up-to-date list, please view the bibliography on PubMed:
Complete Bibliography2024
Sheahan TP, Stevens LJ, Narowski TM, Krajewski TJ, Mollan KR, Gribble J, Moreria FR, Castillo IN, Cuadra E, Alabanza P, Loftis AJ, Coombs RW, Goecker EA, Greninger A, Chappell JD, Won JJ, Lipansky F, Holman W, Szewczyk LJ, Baric R, Painter WP, Eron JJ, Premkumar L, Denison M, Fischer WA. The Antiviral Mechanism of Action of Molnupiravir in Humans with COVID-19. medRxiv. 2023 Nov 27 [Preprint]
Agarwal R, Ha C, Cortes FH, Lee Y, Martínez A, Gálvez RI, Castillo IN, Balmaseda Á, Harris E, Romero-Vivas CM, Premkumar L, Falconar AK, Grifoni A, Sette A, Weiskopf D. Identification of immunogenic and cross-reactive chikungunya virus-specific CD4+ T cell epitopes in chronic chikungunya viral arthritic disease in humans. Cold Spring Harbor Laboratory;bioRxiv. 2024 Oct 25 [Preprint]
Thiono D, Samaras D, Phan TK, Zhu D, Shah R, Castillo IN, Forsberg LJ, Premkumar L, Baric RS, Tian S, Kuhlman B, de Silva AM. Stabilized dengue virus 2 envelope subunit vaccine redirects the neutralizing antibody response to all E-domains. Cold Spring Harbor Laboratory. bioRxiv. 2024 Jul 18 [Preprint]
Chang J, Agarwal R, Côrtes FH, Ha C, Villalpando J, Castillo IN, Gálvez RI, Grifoni A, Sette A, Romero-Vivas CME, Heise MT, Premkumar L, Falconar AKI, Weiskopf D. Chikungunya-virus-specific CD4+ T cells are associated with chronic chikungunya viral arthritic disease in humans. bioRxiv. 2023 Nov 21 [Preprint]
Hein LD, Castillo IN, Medina FA, Vila F, Segovia-Chumbez B, Muñoz-Jordán JL, Whitehead SS, Adams LE, Paz-Bailey G, de Silva AM, Premkumar L. Multiplex sample-sparing assay for detecting type-specific antibodies to Zika and dengue viruses: an assay development and validation study. Lancet Microbe. 2024 Dec 24:100951. doi: 10.1016/j.lanmic.2024.07.014. Epub ahead of print. PMID: 39730005.
Bos S, Zambrana JV, Duarte E, Graber AL, Huffaker J, Montenegro C, Premkumar L, Gordon A, Kuan G, Balmaseda A, Harris E. Serotype-specific epidemiological patterns of inapparent versus symptomatic primary dengue virus infections: a 17-year cohort study in Nicaragua. Lancet Infect Dis. 2024 Oct 25:S1473-3099(24)00566-8. doi: 10.1016/S1473-3099(24)00566-8. Epub ahead of print. PMID: 39489898.Joseph JO, Ylade M, Daag JV, Aogo R, Crisostomo MV, Mpingabo P, Premkumar L, Deen J, Katzelnick LC. High transmission of endemic human coronaviruses before and during the COVID-19 pandemic in adolescents in Cebu, Philippines. BMC Infect Dis. 2024 Sep 27;24(1):1042. doi: 10.1186/s12879-024-09672-8. PMID: 39333882; PMCID: PMC11430261.
Seaman WT, Keener O, Nanayakkara D, Mollan KR, Premkumar L, Cuadra EC, Jones CD, Pettifor A, Bowman NM; UNC OBSc Working Group; Wang F, Webster-Cyriaque J. Oral SARS-CoV-2 host responses predict the early COVID-19 disease course. Sci Rep. 2024 Sep 18;14(1):21788. doi: 10.1038/s41598-024-67504-w. PMID: 39294156; PMCID: PMC11411107.Voss WN, Mallory MA, Byrne PO, Marchioni JM, Knudson SA, Powers JM, Leist SR, Dadonaite B, Townsend DR, Kain J, Huang Y, Satterwhite E, Castillo IN, Mattocks M, Paresi C, Munt JE, Scobey T, Seeger A, Premkumar L, Bloom JD, Georgiou G, McLellan JS, Baric RS, Lavinder JJ, Ippolito GC. Hybrid immunity to SARS-CoV-2 arises from serological recall of IgG antibodies distinctly imprinted by infection or vaccination. Cell Rep Med. 2024 Aug 20;5(8):101668. doi: 10.1016/j.xcrm.2024.101668. Epub 2024 Aug 1. PMID: 39094579; PMCID: PMC11384961.
Mallory M, Munt JE, Narowski TM, Castillo I, Cuadra E, Pisanic N, Fields P, Powers JM, Dickson A, Harris R, Wargowsky R, Moran S, Allabban A, Raphel K, McCaffrey TA, Brien JD, Heaney CD, Lafleur JE, Baric RS, Premkumar L. COVID-19 point-of-care tests can identify low-antibody individuals: In-depth immunoanalysis of boosting benefits in a healthy cohort. Sci Adv. 2024 Jun 14;10(24):eadi1379. doi: 10.1126/sciadv.adi1379. Epub 2024 Jun 12. PMID: 38865463; PMCID: PMC11168476.
Bos S, Zambrana JV, Duarte E, Graber AL, Huffaker J, Montenegro C, Premkumar L, Gordon A, Balmaseda A, Harris E. Inapparent primary dengue virus infections reveal hidden serotype-specific epidemiological patterns and spectrum of infection outcome: a cohort study in Nicaragua. medRxiv [Preprint]. 2024 Apr 8:2024.04.05.24305281. doi: 10.1101/2024.04.05.24305281. PMID: 38633800; PMCID: PMC11023678.
Ciccone EJ, Zhu DR, Gunderson AK, Hawke S, Ajeen R, Lodge EK, Shook-Sa BE, Abernathy H, Garrett HE, King E, Alavian N, Reyes R, Taylor JL, Beatty C, Chung C, Mendoza CE, Weber DJ, Markmann AJ, Premkumar L, Juliano JJ, Boyce RM, Aiello AE. Magnitude and Durability of the Antibody Response to mRNA-Based Vaccination Among SARS-CoV-2 Seronegative and Seropositive Health Care Personnel. Open Forum Infect Dis. 2024 Jan 19;11(1):ofae009. doi: 10.1093/ofid/ofae009. PMID: 38293246; PMCID: PMC10826795.
2023
Joseph JO, Ylade M, Daag JV, Aogo R, Crisostomo MV, Mpingabo P, Premkumar L, Deen J, Katzelnick L. High transmission of endemic human coronaviruses before and during the COVID-19 pandemic in adolescents in Cebu, Philippines. Res Sq [Preprint]. 2023 Nov 16:rs.3.rs-3581033. doi: 10.21203/rs.3.rs-3581033/v1. PMID: 38014070; PMCID: PMC10680936.
Fritch EJ, Mordant AL, Gilbert TSK, Wells CI, Yang X, Barker NK, Madden EA, Dinnon KH 3rd, Hou YJ, Tse LV, Castillo IN, Sims AC, Moorman NJ, Lakshmanane P, Willson TM, Herring LE, Graves LM, Baric RS. Investigation of the Host Kinome Response to Coronavirus Infection Reveals PI3K/mTOR Inhibitors as Betacoronavirus Antivirals. J Proteome Res. 2023 Oct 6;22(10):3159-3177. doi: 10.1021/acs.jproteome.3c00182. Epub 2023 Aug 27. PMID: 37634194.
Tarke A, Zhang Y, Methot N, Narowski TM, Phillips E, Mallal S, Frazier A, Filaci G, Weiskopf D, Dan JM, Premkumar L, Scheuermann RH, Sette A, Grifoni A. Targets and cross-reactivity of human T cell recognition of common cold coronaviruses. Cell Rep Med. 2023 Jun 20;4(6):101088. doi: 10.1016/j.xcrm.2023.101088. Epub 2023 May 29. PMID: 37295422; PMCID: PMC10242702.
Mallory M, Munt JE, Narowski TM, Castillo I, Cuadra E, Pisanic N, Fields P, Powers JM, Dickson A, Harris R, Wargowsky R, Moran S, Allabban A, Raphel K, McCaffrey TA, Brien JD, Heaney CD, Lafleur JE, Baric RS, Premkumar L. Longitudinal Analysis of Humoral and Cellular Immune Response Following SARS-CoV-2 Vaccination Supports Utilizing Point-Of-Care Tests to Enhance COVID-19 Booster Uptake. medRxiv [Preprint]. 2023 Apr 4:2023.04.03.23287498. doi: 10.1101/2023.04.03.23287498. PMID: 37066219; PMCID: PMC10104219.
Boggess KA, Stringer EM, Robinson WR, Munoz MC, Goodnight WH, Rahangdale L, Vora NL, Rosenbaum AJ, Bala V, Ivins A, Narowski TM, Jadi R, Premkumar L. Single-center serological surveillance of SARS-CoV-2 in pregnant patients presenting to labor and delivery. Int J Gynaecol Obstet. 2023 Mar;160(3):874-879. doi: 10.1002/ijgo.14587. Epub 2022 Dec 8. PMID: 36416412.
Tarke A, Zhang Y, Methot N, Narowski TM, Phillips E, Mallal S, Frazier A, Filaci G, Weiskopf D, Dan JM, Premkumar L, Scheuermann RH, Sette A, Grifoni A. Targets and cross-reactivity of human T cell recognition of Common Cold Coronaviruses. bioRxiv [Preprint]. 2023 Jan 5:2023.01.04.522794. doi: 10.1101/2023.01.04.522794. Update in: Cell Rep Med. 2023 May 29;:101088. PMID: 36656777; PMCID: PMC9844015.
2022
Ciccone E, Zhu D, Hawke S, Ajeen R, Gunderson A, Lodge E, Shook-Sa BE, Abernathy H, Garrett H, King E, Markmann A, Premkumar L, Juliano JJ, Boyce RM, Aiello A. The magnitude and durability of the antibody response to mRNA-based vaccination among SARS-CoV-2 seronegative and seropositive healthcare personnel, Open Forum Infectious Diseases, 9, (2022) ofac492.1526, https://doi.org/10.1093/ofid/ofac492.1526
Bartelt LA, Markmann AJ, Nelson B, Keys J, Root H, Henderson HI, Kuruc J, Baker C, Bhowmik DR, Hou YJ, Premkumar L, Cornaby C, Schmitz JL, Weiss S, Park Y, Baric R, de Silva AM, Lachiewicz A, Napravnik S, van Duin D, Margolis DM. Outcomes of Convalescent Plasma with Defined High versus Lower Neutralizing Antibody Titers against SARS-CoV-2 among Hospitalized Patients: CoronaVirus Inactivating Plasma (CoVIP) Study. mBio. 2022 Oct 26;13(5):e0175122. doi: 10.1128/mbio.01751-22. Epub 2022 Sep 22. PMID: 36135380; PMCID: PMC9601237.
Yu ED, Narowski TM, Wang E, Garrigan E, Frazier A, Weiskopf D, Grifoni A, Premkumar L, Antunes R, and Sette A. Immunological memory to Common Cold Coronaviruses assessed longitudinally over a three-year period. Cell Host & Microbe, 2022. doi.org/10.1016/j.chom.2022.07.012.
Puhl AC, Gomes GF, Damasceno S, Fritch EJ, Levi JA, Johnson NJ, Scholle F, Premkumar L, Hurst BL, LeeMontiel F, Veras FP, Batah SS, Fabro AT, Moorman NJ, Yount BL, Dickmander R, Baric R, Pearce KH, Cunha FQ, Alves-Filho JC, Cunha TM, Ekins S. Vandetanib Blocks the Cytokines in SARS-Cov-2 Infected Mice. ACS Omega 2022 https://doi.org/10.1021/acsomega.2c02794.
Lebov JF, Hooper SR, Pugh N, Becker-Dreps S, Bowman NM, Brown LM, MacDonald PDM, Lakshmanane P, Jadi R, Bucardo F, Chevez T, Rodriguez AH, Aleman Rivera TJ. Neurological and neuropsychological sequelae of Zika virus infection in children in León, Nicaragua. Rev Panam Salud Publica. 2022 Jul 20;46:e90. doi: 10.26633/RPSP.2022.90. PMID: 35875321; PMCID: PMC9299389.
Pettifor A, DiPrete BL, Shook-Sa BE, Premkumar L, Kuczynski K, Dittmer D, Aiello A, Wallet S, Maile R, Tan J, Jadi R, Pluta L, de Silva AM, Weber DJ, Kim M, Seña AC, Jones CD. A prospective study of asymptomatic SARS-CoV-2 infection among individuals involved in academic research under limited operations during the COVID-19 pandemic. PLoS One. 2022 Apr 25;17(4):e0267353. doi: 10.1371/journal.pone.0267353. PMID: 35468150.
2021
Adams C, Jadi R, Segovia-Chumbez B, Daag J, Ylade M, Medina FA, Sharp TM, Munoz-Jordan JL, Yoon IK, Deen J, Lopez AL, de Silva AM, Premkumar L. Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines. Emerg Infect Dis. 2021 Dec;27(12):3073-3081. doi: 10.3201/eid2712.211150. PMID: 34808091.
Mollan KR, Eron JJ, Krajewski TJ, Painter W, Duke ER, Morse CG, Goecker EA, Premkumar L, Wolfe CR, Szewczyk LJ, Alabanza PL, Loftis AJ, Degli-Angeli EJ, Brown AJ, Dragavon JA, Won JJ, Keys J, Hudgens MG, Fang L, Wohl DA, Cohen MS, Baric RS, Coombs RW, Sheahan TP, Fischer WA. Infectious Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Virus in Symptomatic Coronavirus Disease 2019 (COVID-19) Outpatients: Host, Disease, and Viral Correlates. Clin Infect Dis. 2021 Nov 23:ciab968. doi: 10.1093/cid/ciab968. Epub ahead of print. PMID: 35022711.
Churiwal M, Lin KD, Khan S, Chhetri S, Muller MS, Tompkins K, Smith J, Litel C, Whittelsey M, Basham C, Rapp T, Cerami C, Premkumar L, Lin JT. Assessment of the Field Utility of a Rapid Point-of-Care Test for SARS-CoV-2 Antibodies in a Household Cohort. Am J Trop Med Hyg. 2021 Nov 24:tpmd210592. doi: 10.4269/ajtmh.21-0592. PMID: 34818625.
Segovia-Chumbez, B., Graham, S. D., Jadi, R. S., de Silva, A. M. and Premkumar, L. (2021). Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening. Bio-protocol 11(10): e4026. DOI: 10.21769/BioProtoc.4026.
Cerami C, Popkin-Hall ZR, Rapp T, Tompkins K, Zhang H, Muller MS, Basham C, Whittelsey M, Chhetri SB, Smith J, Litel C, Lin KD, Churiwal M, Khan S, Rubinstein R, Claman F, Mollan K, Wohl D, Premkumar L, Powers KA, Juliano JJ, Lin FC, Lin JT. Household transmission of SARS-CoV-2 in the United States: living density, viral load, and disproportionate impact on communities of color. Clin Infect Dis. 2021 Aug 12:ciab701. doi: 10.1093/cid/ciab701. Epub ahead of print. PMID: 34383889; PMCID: PMC8436395.
Foo CY A, Thompson PM, Chen S-H, Jadi R, Lupo B, DeRose EF, Arora S, Placentra1 VC, Premkumar L, Perera L, Pedersen LC, Martin N, Mueller GA. The mosquito protein AEG12 displays both catalytic and antiviral properties via a common lipid transfer mechanism. Natl. Acad. Sci. U.S.A (2021) 118 (11) e2019251118; https://doi.org/10.1073/pnas.2019251118
Gawriljuk VO, Zin PPK, Puhl AC, Zorn KM, Foil DH, Lane TR, Hurst B, Tavella TA, Costa FTM, Premkumar L, Bernatchez J, Godoy AS, Oliva G, Siqueira-Neto JL, Madrid PB, Ekins S. Machine Learning Models Identify Inhibitors of SARS-CoV-2. J Chem Inf Model. 2021 61(9):4224-4235. doi: 10.1021/acs.jcim.1c00683. PMID: 34387990.
2020
Katzelnick LC, Narvaez C, Arguello S, Mercado BL, Collado D, Ampie O, Elizondo D, Bustos F, Mercado JC, Latta K, Schiller A, Ojeda S, Sanchez N, Plazaola M, Coloma J, Halloran ME, Premkumar L, Gordon A, Narvaez F, de Silva AM, Kuan G, Balmaseda A, Harris E. Prior Zika virus infection increases future risk of severe dengue disease. Science, (2020) 369(6507):1123-1128. doi: 10.1126/science.abb6143.
Grifoni A, Weiskopf D, Ramirez SI, Mateus J, Moderbacher C, Dan JM, Rawlings SA, Sutherland A, Premkumar L, Jadi R, Marrama D, de Silva AM, Frazier A, Carlin A, Greenbaum JA, Peters B, Krammer F, Smith DM, Crotty S, Sette A. Targets of T cell responses to SARS-CoV-2 coronavirus in humans with COVID-19 disease and unexposed individuals, Cell (2020). Doi: https://doi.org/10.1016/j.cell.2020.05.015
Thomas A, Thiono D, Kudlacek S, Forsberg J, Premkumar L, Tian S, Kuhlman B, de Silva AM, and Metz S. Dimerization of dengue virus E-subunits impacts antibody function and domain focus. J Virol (2020) doi: 10.1128/JVI.00745-20.
2019
Segovia-Chumbez, B., Graham, S. D., Jadi, R. S., de Silva, A. M. and Premkumar, L. (2021). Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening. Bio-protocol 11(10): e4026. DOI: 10.21769/BioProtoc.4026.
Furlong EJ, Kurth F, Premkumar L, Whitten AE and Martin JL. Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase, PmScsC. (2019), Acta Cryst D, 75(3):296-307.
2018
Kudlacek ST, Premkumar L, Metz SW, Tripathy A, Bobkov AA, Payne AM, Graham S, Brackbill JA, Miley MJ, de Silva AM, Kuhlman B (2018) Physiological temperatures reduce dimerization of dengue and Zika virus recombinant envelope proteins. J Biol Chem. 293(23):8922-8933.
2017
Metz S, Gallichotte E, Brackbill JA, Miley MJ, Premkumar L, Baric S, de Silva AM (2017) In Vitro Assembly and Stabilization of Dengue and Zika Virus Envelope Protein Homo-Dimers. Sci Rep 7(1):4524.
Last updated 4/2/2023