My research aims at understanding of molecular mechanisms of immune mediated kidney diseases with emphasis on antineutrophil cytoplasmic autoantibody (ANCA) induced glomerulonephritis (ANCA disease). My recent efforts are focuses on 1) elucidating genetic basis that regulates the pathogenesis of ANCA disease. Recent ongoing studies using MPO-ANCA disease mouse model indicate that anti-MPO IgG caused different severity of ANCA disease in 13 different strains of mice, which mimics disease variation in ANCA patients. Genomic and gene expression microarray studies are underway to identify genes responsible for these differences in disease severity. 2) identifying specific epitopes that are targeted by pathogenic anti-MPO IgG. Recombinant mouse/human MPO chimeric molecules have created and the pathogenic epitopes are being mapped using the chimeric molecules. 3) developing PR3-ANCA disease mouse model. Success with this model would advance our understanding of mechanism of PR3-ANCA disease.
Jennette JC, Xiao H, Hu P. Complement in ANCA-Associated Vasculitis. Semin Nephrol. 2013 Nov; 33(6):557-64.
Xiao H, Ciavatta D, Aylor DL, Hu P, de Villena FP, Falk RJ, Jennette JC. Genetically determined severity of anti-myeloperoxidase glomerulonephritis. Am J Pathol. 2013 Apr; 182(4):1219-1226.
Jennette JC, Falk RJ, Hu P, and Xiao H. Pathogenesis of Antineutrophil Cytoplasmic Autoantibody–Associated Small-Vessel Vasculitis. Annu. Rev. Pathol. Mech. Dis. 2013; 8:139–160.
Yuan Z, Qiao C, Hu P, Li J, Xiao X. A Versatile Adeno-Associated Virus Vector Producer Cell Line Method for Scalable Vector Production of Different Serotypes. Hum Gene Ther. 2011 Mar; 22:613-624.
Wang B, Li J, Qiao C, Chen C, Hu P, Zhu X, Zhou L, Bogan J, Kornegay J, Xiao Xiao. A canine minidystrophin is functional and therapeutic in mdx mice. Gene Therapy. 2008 Apr; 15(15): 1099-1106.
Berkowitz P, Hu P, Warren S, Liu Z, Diaz AL, Rubenstein DS. p38MAPK inhibition prevents disease in pemphigus vulgaris mice. PNAS 2006 Aug; 103(34):12855-12860.
Hu P, Berkowitz P, Madden VJ, Rubenstein DS. Stabilization of plakoglobin and enhanced keratinocyte cell-cell adhesion by intracellular O-glycosylation. J Biol Chem 2006 May; 281(18):12786-12791.
Berkowitz P, Hu P, Liu Z, Diaz AL, Enghild JJ, Chua MP, Rubenstein DS. Desmosome signaling: inhibition of p38MAPK prevents pemphigus vulgaris IgG-induced cytoskeleton reorganization. J Biol Chem. 2005 June; 280(25):23778-23784.
Xiao H, Heeringa P, Liu Z, Huugen D, Hu P, Maeda N, Falk RJ, Jennette JC: The role of neutrophils in induction of glomerulonephritis by anti-myeloperoxidase antibody. Am J Pathol. 2005; 167:39-45.
Hu P, Berkowitz P, O’keefe EJ, Rubenstein DS. Keratinocyte adherens junctions initiate nuclear signaling by translocation of plakoglobin from the membrane to the nucleus. J Invest Dermatol. 2003 Aug; 121:242-251.
Xiao H, Heeringa P, Hu P, Liu Z, Zhao M, Aratani Y, Maeda NN, Falk RJ, Jennette JC. Antineutrophil Cytoplasmic Autoantibodies Specific for Myeloperoxidase Cause Glomerulonephritis and Vesculitis in Mice. J Clin Invest.l 2002 Oct; 110:955-963.
Hu P, O’keefe EJ, Rubenstein DS. Tyrosine phosphorylation of human keratinocyte β-catenin and plakoglobin reversely regulates their binding to E-cadherin and α-catenin. J Invest Dermatol 2001 Nov; 117:1059-1067.