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Microbiome/Metagenome Data Analysis Workshop

May 9, 2022 @ 9:00 am - 4:00 pm

Bioinformatics and Analytics Research Collaborative (BARC), UNC Microbiome core and SOM Office of Research are planning to host a hands-on bioinformatics data analysis workshop focused on “Analysis of next generation sequence data for microbiomes, metagenomes and metatranscriptomes” soon.

Please see the announcement below for information regarding how to apply. An application is required and we will be doing a selection of candidates. We generally are able to select one person per investigator group. If more than one person applies from a lab we may ask the PI to choose one person to attend.


The workshop will discuss the basics of processing 16S rRNA amplicon, metagenomic, and metatranscriptomic sequencing of microbial communities.  For 16S rRNA amplicon sequencing results, students will learn to use QIIME2 to determine taxonomic composition and for preliminary diversity and differential abundance analysis. For metagenomic and metatranscriptomic sequencing results, students will learn to use Kraken2/Bracken and HUMAnN2 to determine composition of taxa, gene families, and pathways.  Microbiome data will be analyzed and visualized in QIIME2 and R. We will also cover metagenome assembly strategies.


When: May 9th through May 11th 2022 (9 am – 4 pm).

Where: UNC Friday Center (Parking will be available)


Points to note:

1. Workshop is only open for researchers with a current affiliation with UNC-CH

2. Workshop will be held at an off-campus location. You should be willing/able to attend for the entire duration of the workshop. Since the sessions build upon sequential learning it may be difficult for you to keep up (and for us to help you) if you miss any sessions.

3. Free Breakfast/Lunch/session breaks are included.

4. There is no charge to attend as of now (Necessary Disclaimer: subject to change/a small fee).

5. You must have a laptop (PC/Mac) that you can bring along.

6. Workshop is limited to 15 (fifteen) participants due to venue space constraints, COVID protocols  and to ensure that everyone gets adequate attention/help.

Application procedure:

If you are interested in attending the workshop, please complete the questionnaire (just the items between the lines below) and send to Dr. Hemant Kelkar (hkelkar@unc.edu) by email by 5 pm on April 25th, 2022.

Applicants will be notified of the selection decision by May 1st.


Please answer the following questions to best of your knowledge.

1. Are you comfortable using linux? If you have no linux experience please indicate that (prior experience is not required/expected).

2. Have you done QIIME2 analysis before? If yes, how comfortable are you with QIIME2 package?

3. Have you done any microbiome related data analysis before? If yes, can you summarize your experience?

4. Do you have your own microbiome/metagenome sequencing dataset you are planning to bring? (if yes, specify if 16S rRNA amplicon or WGS sequencing). If you presently don’t have your own data are you planning to do a related experiment soon?

5. Provide some biographical information.

A. Your Information

Name:

UNC Email:

ONYEN:

Contact phone:

Current UNC Affiliation (student, postdoc, staff, faculty):

B. Information about your PI (if you are not the PI)

Name:

Department:

UNC Email:

Details

Date:
May 9, 2022
Time:
9:00 am - 4:00 pm
Series:
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