Histopathology and Immunohistochemistry
Contents
Histology
CGBID Histology Core
CGBID Histology Core provides a full range of histology services, encompassing routine and specialized grossing, tissue processing, paraffin embedding, microtomy, H&E and special staining, and immunohistochemistry. Consultations are also available. CGIBD members receive priority.
Histology Research Core Facility
The Histology Research Core Facility serves the histology needs of the UNC community and beyond.
We have experience with various organisms including, but not limited to mouse, rat, pig, cat, dog, fish, human and primate. Additionally, we have provided histology services for plants and invertebrates such as coral, caterpillars and even stinkbugs!
Research applications: We take pride in providing top quality paraffin and frozen sectioning, special staining, and both fluorescent and chromogenic single- and multi-label immunohistochemistry. Furthermore, we recently added in situ hybridization using RNAscope and BaseScope technology to our list of services. Our lab houses an extensive slide catalogue for our customer’s use as positive and negative controls, provides access to HIER for antigen retrieval in conjunction with our IHC/IF services, and offers the CryoJane Tape Transfer system to improve morphology during frozen sectioning.
We offer all services at competitive rates with a quick turn-around time of typically within 2 weeks of sample submission. We are here to talk with you at any stage in the research process about your specific research aims and the best way to achieve the results you need.
Please explore our new website where you can find detailed information about all of our services, submission instructions, answers to frequently asked questions and more.
We are located in rooms 212, 213, 214, and 216 in Taylor Hall at 109 Mason Farm Rd Chapel Hill, NC 27514. We can be reached by phone at (919) 966-1202 or e-mail at hrc@med.unc.edu. Please contact us to discuss your specific research needs!
Instrumentation available: Leica 1950 Cryostats, LKB Rotary Microtome, LeicaRM2235 Rotary Microtome, eVos Microscope, RNAScope and HiPlex technology, CryoJane Tape Transfer System for the cryostat, Vibratome,
Eligible users: Any researchers at UNC or other academic institutions, and in industry.
Pathology Service Core
The Pathology Service Core’s animal histopathology component offers consultation and training in necropsy techniques and histopathologic assays, as well as routine histology services such as processing, embedding, sectioning an H&E and special stains.
Research applications: Major services include tissue embedding and sectioning (frozen and paraffin), routine and special stains, consultation on animal study design, tissue collection, immunohistochemistry (IHC), immunofluorescence (IF), and pathology consultation. The core also provides in situ hybridization through RNAScope and BaseScope and spatial biology technologies including 10X Genomics Visium, Akoya Phenocycler, and NanoString GeoMX
Instrumentation available: Leica St5010 Autostainer XL, Leica CV5030 Coverslipper, NX70 Cryostat, Leica Automated Tissue Processor, ASP6025, Fluorescence Microscope E600, Leica RM2255 Automated Microtomes, Histocore Arcadia Embedder, Ventana Discovery Ultra Stainer.
Immunohistochemistry and in situ Hybridization
Histology Research Core Facility
The Histology Research Core Facility serves the histology needs of the UNC community and beyond.
We have experience with various organisms including, but not limited to mouse, rat, pig, cat, dog, fish, human and primate. Additionally, we have provided histology services for plants and invertebrates such as coral, caterpillars and even stinkbugs!
Research applications: We take pride in providing top quality paraffin and frozen sectioning, special staining, and both fluorescent and chromogenic single- and multi-label immunohistochemistry. Furthermore, we recently added in situ hybridization using RNAscope and BaseScope technology to our list of services. Our lab houses an extensive slide catalogue for our customer’s use as positive and negative controls, provides access to HIER for antigen retrieval in conjunction with our IHC/IF services, and offers the CryoJane Tape Transfer system to improve morphology during frozen sectioning.
We offer all services at competitive rates with a quick turn-around time of typically within 2 weeks of sample submission. We are here to talk with you at any stage in the research process about your specific research aims and the best way to achieve the results you need.
Please explore our new website where you can find detailed information about all of our services, submission instructions, answers to frequently asked questions and more.
We are located in rooms 212, 213, 214, and 216 in Taylor Hall at 109 Mason Farm Rd Chapel Hill, NC 27514. We can be reached by phone at (919) 966-1202 or e-mail at hrc@med.unc.edu. Please contact us to discuss your specific research needs!
Instrumentation available: Leica 1950 Cryostats, LKB Rotary Microtome, LeicaRM2235 Rotary Microtome, eVos Microscope, RNAScope and HiPlex technology, CryoJane Tape Transfer System for the cryostat, Vibratome,
Eligible users: Any researchers at UNC or other academic institutions, and in industry.
Pathology Service Core
The Pathology Service Core’s animal histopathology component offers consultation and training in necropsy techniques and histopathologic assays, as well as routine histology services such as processing, embedding, sectioning an H&E and special stains.
Research applications: Major services include tissue embedding and sectioning (frozen and paraffin), routine and special stains, consultation on animal study design, tissue collection, immunohistochemistry (IHC), immunofluorescence (IF), and pathology consultation. The core also provides in situ hybridization through RNAScope and BaseScope and spatial biology technologies including 10X Genomics Visium, Akoya Phenocycler, and NanoString GeoMX
Instrumentation available: Leica St5010 Autostainer XL, Leica CV5030 Coverslipper, NX70 Cryostat, Leica Automated Tissue Processor, ASP6025, Fluorescence Microscope E600, Leica RM2255 Automated Microtomes, Histocore Arcadia Embedder, Ventana Discovery Ultra Stainer.
Spatial Biology
Pathology Service Core
The Pathology Service Core’s animal histopathology component offers consultation and training in necropsy techniques and histopathologic assays, as well as routine histology services such as processing, embedding, sectioning an H&E and special stains.
Research applications: Major services include tissue embedding and sectioning (frozen and paraffin), routine and special stains, consultation on animal study design, tissue collection, immunohistochemistry (IHC), immunofluorescence (IF), and pathology consultation. The core also provides in situ hybridization through RNAScope and BaseScope and spatial biology technologies including 10X Genomics Visium, Akoya Phenocycler, and NanoString GeoMX
Instrumentation available: Leica St5010 Autostainer XL, Leica CV5030 Coverslipper, NX70 Cryostat, Leica Automated Tissue Processor, ASP6025, Fluorescence Microscope E600, Leica RM2255 Automated Microtomes, Histocore Arcadia Embedder, Ventana Discovery Ultra Stainer.
Pathology Review
Pathology Service Core
The Pathology Service Core’s animal histopathology component offers consultation and training in necropsy techniques and histopathologic assays, as well as routine histology services such as processing, embedding, sectioning an H&E and special stains.
Research applications: Major services include tissue embedding and sectioning (frozen and paraffin), routine and special stains, consultation on animal study design, tissue collection, immunohistochemistry (IHC), immunofluorescence (IF), and pathology consultation. The core also provides in situ hybridization through RNAScope and BaseScope and spatial biology technologies including 10X Genomics Visium, Akoya Phenocycler, and NanoString GeoMX
Instrumentation available: Leica St5010 Autostainer XL, Leica CV5030 Coverslipper, NX70 Cryostat, Leica Automated Tissue Processor, ASP6025, Fluorescence Microscope E600, Leica RM2255 Automated Microtomes, Histocore Arcadia Embedder, Ventana Discovery Ultra Stainer.