Research Highlights

A conserved set of mutations for stabilizing soluble envelope protein dimers from Dengue and Zika viruses to advance the development of subunit vaccines.

Phan TTN, Hvasta MG, Kudlacek ST, Thiono DJ, Tripathy A, Nicely NI, de Silva AM, Kuhlman B. J Biol Chem. 2022 May 25:102079. doi: 10.1016/j.jbc.2022.102079. Online ahead of print. PMID: 35643320

Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons.

Sun ZW, Waybright JM, Beldar S, Chen L, Foley CA, Norris-Drouin JL, Lyu TJ, Dong A, Min J, Wang YP, James LI, Wang Y.

Adv Sci (Weinh). 2022 Apr;9(10):e2104317. doi: 10.1002/advs.202104317. Epub 2022 Feb 4. PMID: 35119221 Free PMC article.

Mapping the Morphological Landscape of Oligomeric Di-block Peptide-Polymer Amphiphiles.

Allen BP, Wright ZM, Taylor HF, Oweida TJ, Kader-Pinky S, Patteson EF, Bucci KM, Cox CA, Senthilvel AS, Yingling YG, Knight AS.

Angew Chem Int Ed Engl. 2022 Mar 28;61(14):e202115547. doi: 10.1002/anie.202115547. Epub 2022 Jan 27.

PMID: 35037351

From Protein Design to the Energy Landscape of a Cold Unfolding Protein.

Pulavarti SVSRK, Maguire JB, Yuen S, Harrison JS, Griffin J, Premkumar L, Esposito EA, Makhatadze GI, Garcia AE, Weiss TM, Snell EH, Kuhlman B, Szyperski T.

J Phys Chem B. 2022 Feb 17;126(6):1212-1231. doi: 10.1021/acs.jpcb.1c10750. Epub 2022 Feb 7.

PMID: 35128921

Systematic Variation of Both the Aromatic Cage and Dialkyllysine via GCE-SAR Reveal Mechanistic Insights in CBX5 Reader Protein Binding.

Kean KM, Baril SA, Lamb KN, Dishman SN, Treacy JW, Houk KN, Brustad EM, James LI, Waters ML.

J Med Chem. 2022 Feb 10;65(3):2646-2655. doi: 10.1021/acs.jmedchem.1c02049. Epub 2022 Jan 11.

PMID: 35014255

Functional conservation and divergence of the helix-turn-helix motif of E2 ubiquitin-conjugating enzymes.

Welsh KA, Bolhuis DL, Nederstigt AE, Boyer J, Temple BRS, Bonacci T, Gu L, Ordureau A, Harper JW, Steimel JP, Zhang Q, Emanuele MJ, Harrison JS, Brown NG.

EMBO J. 2022 Feb 1;41(3):e108823. doi: 10.15252/embj.2021108823. Epub 2021 Dec 23.

PMID: 34942047 Free PMC article.

GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses.

Kim SH, Kearns FL, Rosenfeld MA, Casalino L, Papanikolas MJ, Simmerling C, Amaro RE, Freeman R.

ACS Cent Sci. 2022 Jan 26;8(1):22-42. doi: 10.1021/acscentsci.1c01080. Epub 2021 Dec 15.

PMID: 35106370 Free PMC article.

Discovery of Potent Peptidomimetic Antagonists for Heterochromatin Protein 1 Family Proteins.

Lamb KN, Dishman SN, Waybright JM, Engelberg IA, Rectenwald JM, Norris-Drouin JL, Cholensky SH, Pearce KH, James LI, Frye SV.

ACS Omega. 2021 Dec 22;7(1):716-732. doi: 10.1021/acsomega.1c05381. eCollection 2022 Jan 11.

PMID: 35036738 Free PMC article.

Designer proteins that competitively inhibit Gα_q by targeting its effector site.

Hussain M, Cummins MC, Endo-Streeter S, Sondek J, Kuhlman B.

J Biol Chem. 2021 Dec;297(6):101348. doi: 10.1016/j.jbc.2021.101348. Epub 2021 Oct 27.

PMID: 34715131 Free PMC article.

Hexavalent sperm-binding IgG antibody released from vaginal film for development of potent on-demand nonhormonal female contraception.

Shrestha B, Vincent K, Schaefer A, Zhu Y, Vargas G, Motamedi M, Swope K, Morton J, Simpson C, Pham H, Brennan MB, Pauly MH, Zeitlin L, Bratcher B, Whaley KJ, Moench TR, Lai SK.

Proc Natl Acad Sci U S A. 2021 Nov 30;118(48):e2107832118. doi: 10.1073/pnas.2107832118.

PMID: 34815336 Free PMC article.

Alkyl-Aryl Cation Mixing in Chiral 2D Perovskites.

Yan L, Jana MK, Sercel PC, Mitzi DB, You W.

J Am Chem Soc. 2021 Nov 3;143(43):18114-18120. doi: 10.1021/jacs.1c06841. Epub 2021 Oct 21.

PMID: 34672627

Designed, highly expressing, thermostable dengue virus 2 envelope protein dimers elicit quaternary epitope antibodies.

Kudlacek ST, Metz S, Thiono D, Payne AM, Phan TTN, Tian S, Forsberg LJ, Maguire J, Seim I, Zhang S, Tripathy A, Harrison J, Nicely NI, Soman S, McCracken MK, Gromowski GD, Jarman RG, Premkumar L, de Silva AM, Kuhlman B.

Sci Adv. 2021 Oct 15;7(42):eabg4084. doi: 10.1126/sciadv.abg4084. Epub 2021 Oct 15.

PMID: 34652943 Free PMC article.

Coherent control of asymmetric spintronic terahertz emission from two-dimensional hybrid metal halides.

Cong K, Vetter E, Yan L, Li Y, Zhang Q, Xiong Y, Qu H, Schaller RD, Hoffmann A, Kemper AF, Yao Y, Wang J, You W, Wen H, Zhang W, Sun D.

Nat Commun. 2021 Sep 30;12(1):5744. doi: 10.1038/s41467-021-26011-6.

PMID: 34593814 Free PMC article.

Catching COVID: Engineering Peptide-Modified Surface-Enhanced Raman Spectroscopy Sensors for SARS-CoV-2.

Payne TD, Klawa SJ, Jian T, Kim SH, Papanikolas MJ, Freeman R, Schultz ZD.

ACS Sens. 2021 Sep 24;6(9):3436-3444. doi: 10.1021/acssensors.1c01344. Epub 2021 Sep 7.

PMID: 34491043 Free PMC article.

A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2’s Association with the HUSH Complex.

Waybright JM, Clinkscales SE, Barnash KD, Budziszewski GR, Rectenwald JM, Chiarella AM, Norris-Drouin JL, Cholensky SH, Pearce KH, Herring LE, McGinty RK, Hathaway NA, James LI.

ACS Chem Biol. 2021 Sep 17;16(9):1721-1736. doi: 10.1021/acschembio.1c00429. Epub 2021 Aug 20.

PMID: 34415726

Bispecific binder redirected lentiviral vector enables in vivo engineering of CAR-T cells.

Huckaby JT, Landoni E, Jacobs TM, Savoldo B, Dotti G, Lai SK.

J Immunother Cancer. 2021 Sep;9(9):e002737. doi: 10.1136/jitc-2021-002737.

PMID: 34518288 Free PMC article.

Controlling Amphiphilic Polymer Folding beyond the Primary Structure with Protein-Mimetic Di(Phenylalanine).

Warren JL, Dykeman-Bermingham PA, Knight AS.

J Am Chem Soc. 2021 Aug 25;143(33):13228-13234. doi: 10.1021/jacs.1c05659. Epub 2021 Aug 10.

PMID: 34375094

Engineering sperm-binding IgG antibodies for the development of an effective nonhormonal female contraception.

Shrestha B, Schaefer A, Zhu Y, Saada J, Jacobs TM, Chavez EC, Olmsted SS, Cruz-Teran CA, Vaca GB, Vincent K, Moench TR, Lai SK.

Sci Transl Med. 2021 Aug 11;13(606):eabd5219. doi: 10.1126/scitranslmed.abd5219.

PMID: 34380769 Free PMC article.

Inhibition of Isoleucyl-tRNA Synthetase by the Hybrid Antibiotic Thiomarinol.

Johnson RA, Chan AN, Ward RD, McGlade CA, Hatfield BM, Peters JM, Li B.

J Am Chem Soc. 2021 Aug 11;143(31):12003-12013. doi: 10.1021/jacs.1c02622. Epub 2021 Aug 3.

PMID: 34342433

Improved methods for targeting epigenetic reader domains of acetylated and methylated lysine.

Engelberg IA, Foley CA, James LI, Frye SV.

Curr Opin Chem Biol. 2021 Aug;63:132-144. doi: 10.1016/j.cbpa.2021.03.002. Epub 2021 Apr 11.

PMID: 33852996 Review.

Biallelic mutations in the TOGARAM1 gene cause a novel primary ciliopathy.

Morbidoni V, Agolini E, Slep KC, Pannone L, Zuccarello D, Cassina M, Grosso E, Gai G, Salviati L, Dallapiccola B, Novelli A, Martinelli S, Trevisson E.

J Med Genet. 2021 Aug;58(8):526-533. doi: 10.1136/jmedgenet-2020-106833. Epub 2020 Aug 3.

PMID: 32747439

Plant “helper” immune receptors are Ca²⁺-permeable nonselective cation channels.

Jacob P, Kim NH, Wu F, El-Kasmi F, Chi Y, Walton WG, Furzer OJ, Lietzan AD, Sunil S, Kempthorn K, Redinbo MR, Pei ZM, Wan L, Dangl JL.

Science. 2021 Jul 23;373(6553):420-425. doi: 10.1126/science.abg7917. Epub 2021 Jun 17.

PMID: 34140391 Free PMC article.

SPOP and OTUD7A Control EWS-FLI1 Protein Stability to Govern Ewing Sarcoma Growth.

Su S, Chen J, Jiang Y, Wang Y, Vital T, Zhang J, Laggner C, Nguyen KT, Zhu Z, Prevatte AW, Barker NK, Herring LE, Davis IJ, Liu P.

Adv Sci (Weinh). 2021 Jul;8(14):e2004846. doi: 10.1002/advs.202004846. Epub 2021 Jun 1.

PMID: 34060252 Free PMC article.

Discovery of an H3K36me3-Derived Peptidomimetic Ligand with Enhanced Affinity for Plant Homeodomain Finger Protein 1 (PHF1).

Engelberg IA, Liu J, Norris-Drouin JL, Cholensky SH, Ottavi SA, Frye SV, Kutateladze TG, James LI.

J Med Chem. 2021 Jun 24;64(12):8510-8522. doi: 10.1021/acs.jmedchem.1c00430. Epub 2021 May 17.

PMID: 33999620

A Programmable Toolkit to Dynamically Signal Cells Using Peptide Strand Displacement.

Riker KD, Daly ML, Papanikolas MJ, Jian T, Klawa SJ, Shin Sahin JYS, Liu D, Singh A, Miller AG, Freeman R.

ACS Appl Mater Interfaces. 2021 May 12;13(18):21018-21029. doi: 10.1021/acsami.1c03370. Epub 2021 May 3.

PMID: 33938725

DNMT1 reads heterochromatic H4K20me3 to reinforce LINE-1 DNA methylation.

Ren W, Fan H, Grimm SA, Kim JJ, Li L, Guo Y, Petell CJ, Tan XF, Zhang ZM, Coan JP, Yin J, Kim DI, Gao L, Cai L, Khudaverdyan N, Çetin B, Patel DJ, Wang Y, Cui Q, Strahl BD, Gozani O, Miller KM, O’Leary SE, Wade PA, Wang GG, Song J.

Nat Commun. 2021 May 3;12(1):2490. doi: 10.1038/s41467-021-22665-4.

PMID: 33941775 Free PMC article.

Tau seeds are subject to aberrant modifications resulting in distinct signatures.

Tseng JH, Ajit A, Tabassum Z, Patel N, Tian X, Chen Y, Prevatte AW, Ling K, Rigo F, Meeker RB, Herring LE, Cohen TJ.

Cell Rep. 2021 Apr 27;35(4):109037. doi: 10.1016/j.celrep.2021.109037.

PMID: 33910013 Free PMC article.

Biophysical and Structural Characterization of Novel RAS-Binding Domains (RBDs) of PI3Kα and PI3Kγ.

Martinez NG, Thieker DF, Carey LM, Rasquinha JA, Kistler SK, Kuhlman BA, Campbell SL.

J Mol Biol. 2021 Apr 16;433(8):166838. doi: 10.1016/j.jmb.2021.166838. Epub 2021 Feb 1.

PMID: 33539876 Free PMC article.

Modifications to the Framework Regions Eliminate Chimeric Antigen Receptor Tonic Signaling.

Landoni E, Fucá G, Wang J, Chirasani VR, Yao Z, Dukhovlinova E, Ferrone S, Savoldo B, Hong LK, Shou P, Musio S, Padelli F, Finocchiaro G, Droste M, Kuhlman B, Shamshiev A, Pellegatta S, Dokholyan NV, Dotti G.

Cancer Immunol Res. 2021 Apr;9(4):441-453. doi: 10.1158/2326-6066.CIR-20-0451. Epub 2021 Feb 5.

PMID: 33547226 Free PMC article.

BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis.

Fan H, Lu J, Guo Y, Li D, Zhang ZM, Tsai YH, Pi WC, Ahn JH, Gong W, Xiang Y, Allison DF, Geng H, He S, Diao Y, Chen WY, Strahl BD, Cai L, Song J, Wang GG.

Nat Genet. 2020 Dec;52(12):1384-1396. doi: 10.1038/s41588-020-00729-3. Epub 2020 Nov 2.

PMID: 33139953 Free PMC article.

Engineering tetravalent IgGs with enhanced agglutination potencies for trapping vigorously motile sperm in mucin matrix.

Shrestha B, Schaefer A, Chavez EC, Kopp AJ, Jacobs TM, Moench TR, Lai SK.

Acta Biomater. 2020 Nov;117:226-234. doi: 10.1016/j.actbio.2020.09.020. Epub 2020 Sep 13.

PMID: 32937206 Free PMC article.

A High-Throughput Assay to Identify Allosteric Inhibitors of the PLC-γ Isozymes Operating at Membranes.

Huang W, Carr AJ, Hajicek N, Sokolovski M, Siraliev-Perez E, Hardy PB, Pearce KH, Sondek J, Zhang Q.

Biochemistry. 2020 Oct 20;59(41):4029-4038. doi: 10.1021/acs.biochem.0c00511. Epub 2020 Oct 7.

PMID: 33028071 Free PMC article.

Streptavidin Promotes DNA Binding and Activation of cGAS to Enhance Innate Immunity.

Zhang Y, Ma Z, Wang Y, Boyer J, Ni G, Cheng L, Su S, Zhang Z, Zhu Z, Qian J, Su L, Zhang Q, Damania B, Liu P.

iScience. 2020 Aug 14;23(9):101463. doi: 10.1016/j.isci.2020.101463. Online ahead of print.

PMID: 32861998 Free PMC article.

Structural Basis for the Binding Selectivity of Human CDY Chromodomains.

Dong C, Liu Y, Lyu TJ, Beldar S, Lamb KN, Tempel W, Li Y, Li Z, James LI, Qin S, Wang Y, Min J.

Cell Chem Biol. 2020 Jul 16;27(7):827-838.e7. doi: 10.1016/j.chembiol.2020.05.007. Epub 2020 May 28.

PMID: 32470319

Discovery and Characterization of Peptide Inhibitors for Calcium and Integrin Binding Protein 1.

Puhl AC, Bogart JW, Haberman VA, Larson JE, Godoy AS, Norris-Drouin JL, Cholensky SH, Leisner TM, Frye SV, Parise LV, Bowers AA, Pearce KH.

ACS Chem Biol. 2020 Jun 19;15(6):1505-1516. doi: 10.1021/acschembio.0c00144. Epub 2020 May 26.

PMID: 32383857 Free PMC article.

Thermodynamic consequences of Tyr to Trp mutations in the cation-π-mediated binding of trimethyllysine by the HP1 chromodomain.

Krone MW, Albanese KI, Leighton GO, He CQ, Lee GY, Garcia-Borràs M, Guseman AJ, Williams DC Jr, Houk KN, Brustad EM, Waters ML.

Chem Sci. 2020 Mar 4;11(13):3495-3500. doi: 10.1039/d0sc00227e.

PMID: 34109021 Free PMC article.

Physiological temperatures reduce dimerization of dengue and Zika virus recombinant envelope proteins.

Kudlacek ST, Premkumar L, Metz SW, Tripathy A, Bobkov AA, Payne AM, Graham S, Brackbill JA, Miley MJ, de Silva AM, Kuhlman B.

J Biol Chem. 2018 Jun 8;293(23):8922-8933. doi: 10.1074/jbc.RA118.002658. Epub 2018 Apr 20.

PMID: 29678884 Free PMC article.

Bespoke Pretargeted Nanoradioimmunotherapy for the Treatment of Non-Hodgkin Lymphoma.

Au KM, Tripathy A, Lin CP, Wagner K, Hong S, Wang AZ, Park SI.

ACS Nano. 2018 Feb 27;12(2):1544-1563. doi: 10.1021/acsnano.7b08122. Epub 2018 Jan 26.

PMID: 29361211 Free PMC article.

Structural basis for the regulation of β-glucuronidase expression by human gut Enterobacteriaceae.

Little MS, Pellock SJ, Walton WG, Tripathy A, Redinbo MR.

Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E152-E161. doi: 10.1073/pnas.1716241115. Epub 2017 Dec 21.

PMID: 29269393 Free PMC article.

The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3′-end processing.

Aik WS, Lin MH, Tan D, Tripathy A, Marzluff WF, Dominski Z, Chou CY, Tong L.

PLoS One. 2017 Oct 11;12(10):e0186034. doi: 10.1371/journal.pone.0186034. eCollection 2017.

PMID: 29020104 Free PMC article.