The Macromolecular X-ray Crystallography Core has added several new technologies to speed the crystallization of proteins—including membrane proteins—for structure determination.
FRAP for membrane protein crystallization
Structural biology study of membrane proteins is difficult because they often need to be crystallized in lipidic cubic phase (LCP) environments. By using Fluorescence Recovery After Photobleaching (FRAP), you can pre-screen your proteins against a variety of LCP conditions to assess which options foster the best mobility of your protein – a prerequisite for success.
Oryx4 for precision drop-setting and matrix microseeding
We’ve updated our Oryx 4 dropsetter robot to have the fullest capabilities for performing multivariate screening of crystallization conditions while ensuring the most robust sample handling. The Oryx can also perform microseeding experiments for crystal growth and improvement. Plus, it can be configured to set up LCP conditions.
UVEX for dual white light – uv light imaging to find crystal hits
Our JANSi UVEX crystal plate hotel and imager uses an automated microscope to image crystallization experiments in both white light and UV. Comparing pairwise images, experimenters can differentiate between spurious materials and genuine protein crystal leads, resulting in the best use of precious protein samples, streamlined workflows, and the identification of leads that may otherwise have gone overlooked. Your data are available online through a web server so you can monitor your experiments from a distance.
Visit the Macromolecular X-Ray Crysallography Core website for more information about our services