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Mission of the Core Facility

The UNC Metabolomics and Proteomics (MAP) Core provides services for the analysis of proteins and metabolites from tissues, cells or other biological samples. We are available to the UNC community, as well as external academic and industry customers worldwide. We are committed to educating students and researchers in the field of proteomics and metabolomics. We will work with you from initial experimental design through publication. Metabolomics is coming soon! 

We offer an array of sample preparation and instrumentation services, as well as method development and in-depth consultation to help researchers optimize their experimental design based on their experimental goals. The facility is equipped with five state-of-the-art mass spectrometry systems: Thermo QExactive HF, Thermo QExactive HF Biopharma, Thermo Fusion Lumos Tribrid, Thermo Exploris480, and a Thermo Astral. In addition to the mass spectrometers, we have a Thermo Ultimate3000, three Thermo Easy nLC 1200s, an Evosep One, a Vanquish Neo UHPLC, a Vanquish Horizon UHPLC, and a 908 Devices ZipChip capillary electrophoresis device for up-front separation. We have an Agilent 1260 Infinity II HPLC for offline peptide fractionation. We are also now equipped with a Thermo AccelerOme for automated high-throughput sample preparation.

We offer several proteomics services including: protein identification, protein characterization, post-translational modification (PTM) mapping / profiling (for example: phosphoproteomics, acetylomics), large-scale proteomic profiling, intact mass analysis, and affinity purification analyses. We can perform these services quantitatively using labeled (TMT10plex/16plex/18plex) or label-free approaches (DDA or DIA). Comprehensive data analysis is provided through a variety of database search engines and software packages including Proteome Discoverer, MaxQuant, Byos, Spectronaut, and Skyline. We can also aid in bioinformatics analysis using Ingenuity Pathway Analysis (IPA), Gene Ontology, Perseus, and R. Find out more about the services we offer.

Publication Acknowledgement

All work provided by the MAP Core facility which is included in a publication should acknowledge the contribution of the core facility either in the acknowledgements section or as an author. Proper acknowledgment of the core facility enables us to obtain financial and other support so that we may continue to provide essential services in the best ways possible. Please follow ABRF guidelines.