LC-MS/MS systems for Metabolomics

The Fusion Lumos mass spectrometer, featuring Advanced Quadrupole Technology, is equipped with a dual pressure linear ion trap and an ultra-high-field Orbitrap mass analyzer, making it a powerful instrument for metabolomics studies. This high-resolution instrument enables sensitive, high throughput characterization and quantification of metabolites and lipids. Its MSn capabilities and multiple fragmentation methods (CID, HCD, ETC) allow for compressive structural elucidation.

The Thermo Scientific Exploris 240 is a high-resolution, accurate-mass (HRAM) Orbitrap mass spectrometer designed for a broad range of applications, including small molecule analysis and metabolomics. It offers up to 240,000 resolving power, which enhances sensitivity and selectivity for complex sample analyses. This system integrates Thermo’s latest technology for fast, reproducible, and accurate mass detection, making it suitable for both targeted and untargeted workflows. With AcquireX intelligent data acquisition workflows, the Exploris 240 automates MS2 data collection, including low-abundance ions, and minimizes redundancy with dynamic background exclusion. The EASY-IC™ (internal calibration) ion source is an internal calibration system that delivers sub-ppm mass accuracy by automatically introducing an internal reference mass during sample analysis. This feature ensures high mass accuracy, which is crucial for the reliable identification and quantification of analytes, especially in complex samples.

The Thermo Scientific Stellar mass spectrometer integrates a quadrupole mass analyzer for precursor ion selection with a dual-pressure linear ion trap analyzer, achieving high-speed MSn data acquisition up to 140 Hz. The ion concentrating routing multipole (ICRM) enables efficient ion management between the two analyzers, resulting in high sensitivity, a wide dynamic range, and enhanced specificity. This allows for the rapid validation of biomarkers and high-throughput, targeted metabolomics. The instrument’s flexible design supports rapid polarity switching (5 ms) between positive and negative ionization modes and acquires full-scan MS and MS2 spectra with both HCD and CID fragmentation for comprehensive molecular profiling.