LC-MS/MS systems for Proteomics

Thermo’s latest mass spectrometer, the Orbitrap Astral, significantly enhances analytical capabilities by delivering higher throughput, deeper coverage, and increased sensitivity. Paired with either the Vanquish Neo UHPLC or the Evosep One, this powerful system is routinely used for large-scale proteomic studies, including plasma analyses from extensive sample cohorts. The Orbitrap Astral, primarily operated in Data Independent Acquisition (DIA) mode, combines ultra-high resolving power with improved ion transmission efficiency, enabling the precise detection and quantification of low-abundance proteins.

The Exploris 480, paired with a Thermo Ultimate 3000 nanoLC and equipped with a Thermo Flex Ion source, is a powerful system routinely employed for large-scale proteomic analyses. Renowned for its exceptional sensitivity, the Exploris 480 delivers precise quantification capabilities. It is primarily operated with Data Independent Acquisition (DIA) but is also used for Tandem Mass Tag (TMT) based quantitative analysis. Furthermore, the system features SureQuant technology, designed for targeted proteomics.

The Q Exactive HF, paired with a Thermo Easy nLC 1200 and a Thermo Easy Spray source, is an advanced system routinely employed for small to medium-scale proteomic analyses and single protein studies, including protein ID, PTM (Post Translational Modification) site mapping, and protein characterization projects. This system also features parallel reaction monitoring (PRM) for targeted quantitation.