Equipment

Pictured below is the Tecan Freedom Evo®, our largest automated liquid handling system; this unit is linked on the right to the Roche Lightcycler® 480 II. More information about the automated system and pcr machines can be found on company websites:

www.tecan.com/platform/apps/product/index.asp

www.roche-applied-science.com/lightcycler-online/

This video published by the Journal of Visualized Experiments shows how samples may be set up and aliquoted by the Tecan Freedom Evo®, as well as quantified by the Roche Lightcycler® 480 II.

www.jove.com/index/embed.stp

Chugh P, Tamburro K, Dittmer DP (2010). Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays. J Vis Exp. 46. http://www.jove.com/index/details.stp?id=2210, doi: 10.3791/2210

Tecan

 

Below is a closer view of the stage of the Freedom Evo®. The arm is located at the far right top; after a PCR reaction has been set up, the arm moves the plates into the hotel, which has the capacity to store 18 plates. The plates are then moved from the hotel to the Lightcycler® (on the right).

Tecan Freedom Evo Inside

 

Pictured below is the GS Junior system.

GS JuniorDongmei@sequencingbench