For each validated assay we have: standard operating procedures, quality controls built in, and trained personnel. Please see below for a few publications that have resulted from each assay.
Our individual viral arrays include the following viruses: KSHV, EBV, HSV-1, HSV-2, RRV, and HCMV. We also offer a Viral Load assay that includes KSHV, EBV, CMV, and HSV-1 all in one assay.
- KSHVv2.0: 192 primers per sample. (2x rate 4), Host Cell Microbe. 2013, J Infect Dis. 2011, Cancer Res. 2003
- KSHVv400bp: 96 primers per sample. (1x rate 4) unpubl.
- EBVv2.0: 96 primers per sample (1x rate 4) J Virol. 2013, J Virol. 2009, Blood. 2005, Nucleic Acids Res. 2005
- HSV1v1.0: 2x96 primers per sample (1x rate 4) unpubl.
- HSV2v1.0: unpubl.
- RRVv1.0: 96 primers per sample (1x rate 4) Virol. 2009, J Virol. 2005
- HCMVv1.0: 192 primers per sample (2x rate 4) unpubl.
- Viral Load: 5 primers (HSV, KSHV, CMB, and EBV) (5x rate 1). Unpubl.
Our microRNA assays include both in-house developed and commercially available assays. Please contact us for rates as they depend on company and number of miRNAs you desire.
- Pre-miRNAv1.0: 192 primers per sample. In-house assay (2x rate 4) http://www.jove.com/index/details.stp?id=2210, J Vis Exp. 2010, PLoS Pathog. 2009, Blood. 2008
- TaqMan® MicroRNA Assays: Human library purchased from Life Technologies, run on our Tecan and LC480 Instruments. PLoS Patho. 2013, Am J Respir Crit Care Med. 2011, Blood. 2009
- KSHV TaqMan® viral miRNA Assays: Customizable from Life Technologies. J Virol. 2013, PLoS Patho. 2013, PLoS Pathog. 2009,
- microRNA Ready-to-Use PCR panels: miRNA Human Panel and Mouse Panel has been used so far. Panels purchased from Exiqon, run on our Tecan and LC480 Instruments.
Other assays include: NFkappaB and P53response.
Analysis of the raw qPCR is not included in the assay cost; however, we do offer a data analysis/consultation service that can assist you with analysis or creating ready-to-publish tables. Please contact Dr. Dirk Dittmer and/or the Vironomics Core Manger for this service and estimated turn-around time. BMC Bioinform. 2010