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Agilent TapeStation

2019: This service is included in all sequencing projects conducted at the UNC Vironomics core and contained in the library preparation rate. It is no longer available by itself!

Sample submission: Please specify which option you want below AND at submission if any special post-analysis (zoom to sample, specific size regions)

High Sensitivity (HS) D1000 DNA:

  • Double-stranded DNA
  • assess 35-1000bp
  • Concentration-Minimum 5pg/µL. Dilute to below 1000pg/µL (assess by NanoDrop).
  • 5µL if <15 samples, in tubes
    • OR 2µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).

High Sensitivity (HS) D5000 DNA:

  • Double-stranded DNA
  • assess 100-5000bp
  • Concentration-Minimum 5pg/µL. Dilute to below 1000pg/µL (assess by NanoDrop).
  • 5µL if <15 samples, in tubes
    • OR 2µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).

D1000 DNA:

  • Double-stranded DNA
  • assess 35-1000bp
  • Concentration-Minimum 0.1ng/µL. Dilute to below 50ng/µL (assess by NanoDrop).
  • 5µL if <15 samples, in tubes
    • OR 1µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).

RNA:

  • Total RNA. Please specify if prokaryotic or eukaryotic (viral ~eukaryotic)
  • Concentration-Minimum 5ng/µL. Dilute to below 500ng/µL (assess by NanoDrop).
  • 3µL if <15 samples, in tubes
    • OR 1µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).