BRIC Small Animal Imaging Core presents: Cryo-Fluorescence Tomography, A Technique to Bridge Multi-Resolution Multi-Modal Assays

Many human cancer cell lines have been engineered to express fluorescence so that in vivo imaging can be used to monitor and stage disease progression. Following in vivo imaging, traditional histo-pathology can be performed to validate in vivo measurements. However, a gap in sensitivity and resolution between in vivo and ex vivo techniques may make it hard to characterize an animal model. Traditional ex vivo techniques only focus on small sample sizes while allowing for high-resolution evaluation and characterization. Using a Cryo-Fluorescence Tomography (CFT) imaging approach, an imaging modality based on serial slicing and off-the-block fluorescence imaging, we can now bridge the gap between in vivo and ex vivo resolution of the entire animal. This new technique and imaging procedures will be described with various research examples.

SpeakerL Mohammed Farhoud, EMIT Imaging, Inc.

Snacks and coffee will be served