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Zeiss 800 Upright Confocal Microscope

A laser scanning confocal mounted on an Upright Axio Imager .Z2 microscope

Features

  • Useful for cleared tissue, zebrafish, any wet preps with dipping lenses, and general fixed specimens
  • The emission path uses variable dichroic mirrors to achieve flexibility of bandpass adjustment similar to spectral detectors that use a prism or a diffraction grating.
  • The upright configuration of the scope allows the use of wet preparations imaged with dipping objectives.


Location: 231 Taylor Hall


Specifications

Objectives

  • 5x/0.25, WD 12.5mm
  • 10x/0.3 WD 5.2mm
  • 20x/0.8 WD 0.55mm
  • 25x/0.8 multi immersion WD 0.57mm
  • 40x/1.3 oil WD 0.21mm
  • 63x/1.4 oil WD 0.14mm

Excitation Lines

  • 405nm
  • 488nm
  • 561nm
  • 633nm

Epifluorescence Filters

  • DAPI
  • GFP
  • Rhod

Detection

  • 3 GaAsP Detectors
  • 1 TD (transmitted light detector)