Olympus Fluoview 1000 Confocal

A confocal laser scanning microscope mounted on an inverted IX-81 stand.

Features

• A synchronized SIM Scanner allowing for coordination of laser stimulation and imaging, an ideal choice for FRAP, FLIP and photoactivation
• Environmental chamber for maintaining living cells
• Tiling and imaging multiple locations
• CFP/YFP FRET

Location: 238 Taylor Hall

Specifications

Objectives

• UPlanSApo 4x/0.16 WD 13mm
• UPlanSApo 10x/0.4 WD3.1mm
• UPlanSApo 20x/0.75 WD 0.55mm
• UPlanSApo 30x/1.05 Silicon WD 0.880mm
• PlanApo 60x/1.42 oil WD 0.150mm
• LUMPlanF IN 60x/1.00 water WD 2mm

Excitation Lines

• 405nm: 50mW
• 559nm: 15mW
• 635nm:20mW
• Multiline Argon laser (440nm, 488nm, 515nm, Total 30mW)

Epifluorescence Filters

• DAPI
• FITC/GFP
• Texas Red/Rhodamine

Detection

• 4 Confocal Channels & TD (transmitted light detector), Ch1 & Ch 2 are spectral, Ch3 & Ch 4 use barrier filters