The National Gnotobiotic Resource Center is a unique and essential resource for all investigators to study physiologic and patho-physiologic functions of normal and dysbiotic resident bacteria, with particular emphasis on gene-environmental interactions in genetically altered gnotobiotic mice (transgenic, knockout or spontaneously mutated) with altered physiology and disease phenotypes.

In the past 5 years of funding, the NGRRC provided 8279 gnotobiotic mice to 101 investigators in 49 institutions. 68 funded NIH grants (in 12 Institutes) by 51 investigators as well as 63 grants by other funding agencies depend on gnotobiotic mice from the NGRRC.

The specialized features of our facility are:

1) We provide gnotobiotic mice nationally;

2) We have a highly experienced team of technical staff who perform experimental techniques;

3) We provide external investigators the option for onsite tissue collection;

4) We collaborate with the UNC Mutant Mouse Regional Resource Center (MMRRC) and Collaborative Cross (CC) Consortium;

5) We train and support external investigators to set up their own local gnotobiotic units.

Objectives

  • Provide germ-free and gnotobiotic mice to investigators across the country;
  • Derive mouse strains into germ-free conditions;
  • Support new investigators to generate preliminary data for grant applications;
  • Train personnel to develop gnotobiotic facilities at other institutions;
  • Develop and characterize complex microbial communities and libraries of bacterial strains.