Training
The NGRRC provides hands-on training based on your needs. Training sessions range from 1 to 4 days depending on your needs.
During the training experience, you will have the opportunity to learn about:
Contents
Topics Covered
General building setup
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Rooms
- Supply rooms
- Perishable items
- Temperature and humidity parameters
- Nonperishable items
- Perishable items
- Animal room
- Temperature and humidity parameters
- Supply rooms
-
Cage Wash
-
Autoclave
- Importance of steam
- Cycles
- Parameters
- Sterility verification
- Different methods of verification
- Gravity vs. Liquid
- Different methods of verification
Sterilization methods
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Steam
-
EtO
-
Liquid
-
Discussion of when the methods are used for supplies
Building cylinders
-
Supply packing
- Supply limitations for optimal sterility success
-
Placing mylar and tape
-
HEPA filter replacement
- Life expectancy
Isolator building
-
Preparing supplies
- Autoclave isolator shelves and cage components
- Prepping inlet and outlet filters
-
Assembly procedures
- Isolator integrity verification
-
Liquid sterilization
- Commonly used cold sterilant
- Application procedures
-
Basic internal setup
-
Timeline
- Start to finish
- When mice can be introduced
- Start to finish
Reprocessing isolator techniques
-
Disinfection
- Commonly used cold sterilant
- Application procedures
-
Disassembly
-
Rebuilding
- Verification of the potential reuse of isolator components
- What can be reused vs. what can’t
- Review of assembly procedures
- Verification of the potential reuse of isolator components
-
Isolator sterility verification
- Quality Control (QC) sampling
- Frequency
- Daily
- Quarterly
- Methods
- Methods for verification
- Sheep Blood Agar
- Thioglycolate broth
- Gram Stain
- 16s PCR
- Methods for verification
- Frequency
- Quality Control (QC) sampling
-
Cage and isolator cleaning
- Standard Operation Procedure (SOP) overview
- Observation of staff
-
Animal shipment and receiving procedures
- SOP overview