Examples of Our Seq Runs
Here we will keep examples of our Ion Torrent sequencing runs and metrics, click for larger images
Examples of our runs on Ion Torrent S5
(Click for larger image)
~200bp S5 run 102 million reads!
(poly(A) RNAseq) 540 chip
~400bp S5 run 19 million reads!
530 chip
~600bp S5 run 11 million reads!
530 chip
PHRED/Q Scores
PHRED(33)/Q Scores are a measure of accuracy at each base position. It isn’t enough to say we sequenced 6.5 million reads (1 sample) from a 600bp fragment protocol if by the 600th base-pair the sequence is unreliable; luckily our Ion Torrent S5 sequencing is producing great base-pair qualities all the way to the end.
Figures produced on Qiagen CLC Bio Genomics Workbench software by Jay Zhang
LEGEND
PHRED(33)/Q Score | Incorrect | Accuracy |
---|---|---|
10 | 1 in 10 | 90% |
20 | 1 in 100 | 99% |
30 | 1 in 1000 | 99.9% |
40 | 1 in 10,000 | 99.99% |