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Here we will keep examples of our Ion Torrent sequencing runs and metrics, click for larger images

Examples of our runs on Ion Torrent S5

(Click for larger image)

June 3, 2017 by Avery Cheves
~200bp S5 run 102 million reads!
(poly(A) RNAseq) 540 chip

19,034,414 reads!Aug 9, 2016(Technician: Avery)
~400bp S5 run 19 million reads!
530 chip

Best 600bp run by technician Jay Zhang, March 31, 2017 ~600bp S5 run 11 million reads!
530 chip


PHRED/Q Scores

PHRED(33)/Q Scores are a measure of accuracy at each base position. It isn’t enough to say we sequenced 6.5 million reads (1 sample) from a 600bp fragment protocol if by the 600th base-pair the sequence is unreliable; luckily our Ion Torrent S5 sequencing is producing great base-pair qualities all the way to the end.

Figures produced on Qiagen CLC Bio Genomics Workbench software by Jay Zhang

Vironomics Ion Torrent S5 600bp PHRED Score, CLC. (Barcode 1, 6.5M reads, technician Jay Zhang, 3/31/17)

Vironomics Ion Torrent S5 PHRED ave Quality scores for 600bp, CLC. (Barcode 1, 6.5M reads, technician Jay Zhang, 3/31/17)

LEGEND

PHRED(33)/Q Score Incorrect Accuracy
10 1 in 10 90%
20 1 in 100 99%
30 1 in 1000 99.9%
40 1 in 10,000 99.99%