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UNC School of Medicine

Agilent TapeStation

This service is included in all sequencing projects conducted at the UNC Vironomics core and contained in the library preparation rate. It is no longer available by itself!

Sample submission: Please specify which option you want below, and at submission if any special post-analysis (zoom to sample, specific size regions)

High Sensitivity (HS) D1000 DNA:

  • Double-stranded DNA
  • assess 35-1000bp
  • Concentration-Minimum 5pg/µL. Dilute to below 1000pg/µL (assess by NanoDrop).
  • 5µL if <15 samples, in tubes
    • OR 2µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).

High Sensitivity (HS) D5000 DNA:

  • Double-stranded DNA
  • assess 100-5000bp
  • Concentration-Minimum 5pg/µL. Dilute to below 1000pg/µL (assess by NanoDrop).
  • 5µL if <15 samples, in tubes
    • OR 2µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).

D1000 DNA:

  • Double-stranded DNA
  • assess 35-1000bp
  • Concentration-Minimum 0.1ng/µL. Dilute to below 50ng/µL (assess by NanoDrop).
  • 5µL if <15 samples, in tubes
    • OR 1µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).

RNA:

  • Total RNA. Please specify if prokaryotic or eukaryotic (viral ~eukaryotic)
  • Concentration-Minimum 5ng/µL. Dilute to below 500ng/µL (assess by NanoDrop).
  • 3µL if <15 samples, in tubes
    • OR 1µL if >15-96 samples, in special plate (if local, arrange to get plate from the Core first. Not local, send in tubes).