(Rate 1) Viral load batch 8 experimental:

The rate is per sample (batch of 8) per real-time PCR primer. The core does not accept single samples for this rate. This rate will determine virus loads, i.e. how much virus is in a supplied sample. The Core will provide a central service for quantitative single viral load determination (using real-time QPCR) of experimental samples, this includes running quality control of reagents. This excludes clinical material.

(Rate 2) Viral load batch 8 set-up:

The rate is annually per sample virus. This is a set-up fee, which is due annually for each new virus that the core will quantitate using rate 1. This set-up fee covers necessary regulatory, quality-control, validation and data set-up work. The Vironomics Core Facility will maintain an infrastructure that is compliant with these regulations and perform the administrative action needed to be in compliance. These may differ depending on the particular virus.

(Rate 3) Viral Load (clinical):

The rate is per sample. This rate will determine virus loads, i.e. how much virus is in a supplied sample. This rate covers sample genomic isolation, cDNA preparation if needed, and analysis of this sample with a single primer and control for sample integrity.. The reporting and regulatory requirements are much more stringent for clinical material. Clinical samples require a heavy investment to conform with federal standards that apply to biosafety, GLP, HIPPA and GCLP regulations. Note that the core does not accept patient samples, or any samples that can be linked to individual persons. All samples must be de-identified

(Rate 4) 96 primer array:

The rate is per sample and provides for the analysis of this sample, in duplicate, with 96 different primers using in-house developed arrays (IP contributed by the Dittmer group). This rate only applies to non-clinical samples. The Core Facility will provide microarray services based on real-time QPCR. At this point the core is offering the following UNC-developed arrays: KSHV, KSHV, EBV, HSV-1, HCMV, RRV, NFkB, p53, TLR, StemCell, pre-miRNA. This technology is more sensitive, more specific and targeted to specific needs of the user. This is in contrast to hybridization-based microarray facilities, which only use commercial assays to profile gene expression.

(Rate 5) 96 primer array set up:

The rate is annually per sample array. This is a set-up fee, which is due annually for each new virus that the core will quantitate using rate 4. The rate only applies to non-clinical samples. This set-up fee covers necessary regulatory, quality control, validation and data set-up work.. The Vironomics Core Facility will maintain an infrastructure that is compliant with these regulations and perform the administrative action needed to be in compliance. These may differ depending on the particular array.

(Rate 6) 96 gene viral array (clinical):

The rate is per sample and provides for the sample genomic isolation, cDNA preparation, and analysis of this sample with 96 different primers using in-house developed arrays (IP contributed by the Dittmer group). This rate applies to clinical samples. Clinical samples require a heavy investment to conform with federal standards that apply to biosafety, GLP, HIPPA and GCLP regulations. Note that the core does not accept patient samples, or any samples that can be linked to individual persons. All samples must be de-identified

(Rate 7) Commercial 96 (4) QCPR:

The rate is per batch of 4 samples. It uses a commercial reagents only rather than “Home-brew”. This rate only applies to non-clinical samples.

(Rate 8) Commercial 96 QCPR set up:

The rate is annually per sample per user of commercial arrays (rate 7). This set-up fee covers necessary regulatory, quality control, validation, robot and data set-up work. The Vironomics Core Facility will maintain an infrastructure that is compliant with these regulations and perform the administrative action needed to be in compliance. These may differ depending on the particular array.

(Rate 9) Data analysis:

The rate is per hour. The core will provide data analysis for users, who do not wish to do it themselves. As with all core technologies, data analysis is not straightforward.

Roche 454 Junior Sequencing Library:

This rate is per sample preparation. This rate covers reagents, quality control checks, reagent procurement, equipment, bench space, and protocol assistance by staff. Customer is responsible for actually doing the sample library preparation. The Core will provide a central service for preparing samples for next generational sequencing using the Roche 454 GS Junior. As staffing allows, customers may submit samples and request for the Vironomics Core to perform Libraries for them, this will incur an additional 4 hours of data analysis rate 9. Inquire for pricing.

Roche 454 Junior Sequencing emPCR:

This rate is per sample preparation. This rate covers reagents, quality control checks, reagent procurement, equipment, bench space, and protocol assistance by staff. This includes quantification and quality check by Agilent and Picogreen reagents also. Customer is responsible for actually doing the sample Agilent, Picogreen, and emPCR preparation. The Core will provide a central service for preparing samples for next generational sequencing using the Roche 454 GS Junior. As staffing allows, customers may submit samples and request for the Vironomics Core to perform emPCR for them, this will incur an additional 8 hours of data analysis rate 9. Inquire for pricing.

Roche 454 Sequencing:

This rate is per sequencing PicoTiter Plate set-up and run. This rate includes Core staff setting-up and running the PicoTiter Plate and Roche 454 GS Junior Sequencer. This also includes reagents, reagent procurement, quality control checks, equipment, final data prepared by data analyst for customer, and two years of data storage. The Core will provide a central service for sequencing samples for next generational sequencing using the Roche 454 GS Junior. Inquire for pricing.