The Animal Models Core offers a full suite of genome engineering services for the generation of genetically modified mouse and rat models for biomedical research.
The Animal Models Core has fully implemented the CRISPR/Cas9 system for genome modification in mice, rats and cell lines.
The Animal Models Core can produce knockout mice, conditional knockout mice, knockin mice, etc. by gene targeting in embryonic stem cells. We offer full-service packages as well as ad-hoc services to meet our clients’ needs.
The Animal Models Core offers a full range of pronuclear injection services for production of transgenic mice. Transgenic mouse production is routinely performed in C57BL/6, with other strains such as FVB or hybrid strains available upon request. Transgenic injections can also be performed in custom strains provided by the client. Transgene cloning and BAC recombineering services are available for production of transgenes.
The Animal Models Core offers a full range of rat genetic modification services including pronuclear microinjection for production of transgenic rats using plasmid or BAC transgenes and production of knockout/knockin rats by injection of CRISPR/Cas9 nucleases.
The Animal Models Core offers a full range of BAC recombineering services
We offer reproductive services including sperm and embryo cryopreservation, strain resuscitation from frozen embryos, strain rederivation, in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Contact the core director for more information.