The Department of Chemistry Mass Spectrometry Core Laboratory is excited to offer mass spectrometry based lipidomics to the UNC Community. With Method Development Grant funding from the Core Facilities Advocacy Committee (CFAC), we have developed mass spectrometry based methods for the extraction, chromatographic separation, detection and data processing of lipids from biological matrices. Our methods target a diverse array of lipid compound classes to allow for comprehensive sample profiling. We can provide lipid identification and/or relative quantitation methods for your experimental needs.
Data are generated via LC-MS methods with our Waters Acquity LC system coupled to a Q Exactive HFX mass spectrometer. The system provides high resolution and accurate mass detection of lipid compounds. We utilize LipidSearch™ software for lipid identification, spectral matching, and relative quantitation.
The core also offers other mass spectrometry platforms to allow for the analysis of volatile lipids and absolute quantification of lipid species, and is equipped with a BSL 2 laboratory for biological hazard sample handling.
The UNC Department of Chemistry Chemical Research Instrumentation Teaching and Core Laboratories, CRITCL, Mass Spectrometry Core laboratory specializes in small molecule analysis. Our services include, but are not limited to:
- complex mixture analysis using chromatography MS
- lipidomics (profiling and quantitation)
- metabolomics
- molecular formula confirmation using accurate mass
- small molecule quantitation using chromatography (gas or liquid) coupled to mass spectrometry (MS).
- structural elucidation using MS/MS and MSn
The laboratory provides individualized user training for undergraduate, graduate, and post-doctoral scholars of the Department of Chemistry and UNC campus. The laboratory aims to provide the highest quality mass spectrometric services possible to its users and clients.
Visit the Department of Chemistry Mass Spectrometry Core website for more information