This is a presentation on the comparison of the same series of libraries run on both the HS2500 and the Novaseq. These were TruSeq stranded mRNA. Pools of 4 libraries were run on the HiSeq 2500/ paired end/ 50x. The same pools were then pooled into larger pools (pools of pools) to be run on the NovaseqS1/ PE/50x to achieve similar data coverage. This presentation covered the differences in the platforms, the data yielded from each and lessons learned.

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