Kavli NSI iDISCO Workshop 2025 resource list
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Tissue preparation
- Official iDISCO+ website (protocol, recommended antibodies, and more)
- AdipoClear:
- Tissue clearing tips and troubleshooting presentation (courtesy of Alba Vieites Pardo)
- Recommended (rabbit) antibody for cFos mapping only [preferred]
- Recommended (rat) antibody for cFos mapping in combination with another antibody
- Lists of validated antibodies:
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Imaging
- Imaging cleared tissue with light-sheet microscopy
- UltraMicroscope II user guide: HTML, PDF (3D-printed holders at the bottom of the PDF download page)
- UltraMicroscope II video tutorials for usage and maintenance.
- UltraMicroscope II step-by-step instructions
- ClearMap and Tubemap suggested imaging settings
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Visualization with Imaris Viewer and analysis with Imaris
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Visualization and analysis with Clearmap
- A fantastic guide to python environments (created by Talley Lambert, Harvard) ; videos and slides version here.
- [DEPRECATED] Installation instructions for Linux (working as of 20230504)
- Clearmap GitHub page
- Original Clearmap test datasets from Renier lab (do not require stitching)
- Allen Brain Atlas download site
- Datasets from workshop (coming soon)
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Data management