Resources

MSL Training materials

MSL’s YouTube channel

Microscope and software video tutorials, lectures

Step-by-step instructions

• Zeiss LSM900
• Zeiss LSM700/LSM710
• UltraMicroscope II light-sheet
• How to flatfield color images
• Tiling and stitching on IX81 (brightfield)

PowerPoint Presentations

• Intro to fluorescence microscopy
• Confocal microscopy
• Confocal microscopy tips
• Light-sheet microscopy
• Light microscope brightfield alignment

Useful guides and reviews

• Confocal microscopy review
• UltraMicroscope II light-sheet guide (HTML, PDF)
• Tissue Clearing review
• MSL’s recommendations when writing Materials and Methods
• MSL’s light microscopy sample preparation guidelines
• MSL’s recommendations for sampling
• MSL’s recommendations for file formats when saving
• MSL’s recommendations for saving and moving large data files (typically on the light-sheet or Dragonfly)
• Phototoxicity review (essential reading for folks doing live imaging)

MSL’s FIJI Macros

Other useful resources

Other imaging cores on UNC’s campus

• Neuroscience Microscopy Core
• Hooker Imaging Core
• Biology Microscopy Core

Kavli NSI iDISCO Workshop 2023 resource list

Online resources

• iBiology lectures on microscopy
• Molecular expressions – exploring the world of optics and microscopy
• Olympus microscopy resource center
• Nikon MicroscopyU
• Zeiss microscopy online campus
• Imaris homeschool

Good books ($)

• Fundamentals of Light Microscopy and Electronic Imaging
• Quantitative Imaging in Cell Biology

Great courses ($$$)

• Analytical & Quantitative Light Microscopy (Woods Hole, MA)
• Optical Microscopy & Imaging in the Biomedical Sciences (Woods Hole, MA)
• Quantitative Imaging: From Acquisition to Analysis (Cold Spring Harbor, NY)
• Quantitative Fluorescence Microscopy (Bar Harbor, ME)

Maintenance instructions

• UltraMicroscope II alignment instructions