Resources
MSL Training materials
MSL’s YouTube channel
Microscope and software video tutorials, lectures
Step-by-step instructions
- Zeiss LSM900
- Zeiss LSM700/LSM710
- UltraMicroscope II light-sheet
- How to flatfield color images
- Tiling and stitching on IX81 (brightfield)
PowerPoint Presentations
- Intro to fluorescence microscopy
- Confocal microscopy
- Confocal microscopy tips
- Light-sheet microscopy
- Light microscope brightfield alignment
Useful guides and reviews
- Confocal microscopy review
- UltraMicroscope II light-sheet guide (HTML, PDF)
- Tissue Clearing review
- MSL’s recommendations when writing Materials and Methods
- MSL’s light microscopy sample preparation guidelines
- MSL’s recommendations for sampling
- MSL’s recommendations for file formats when saving
- MSL’s recommendations for saving and moving large data files (typically on the light-sheet or Dragonfly)
- Phototoxicity review (essential reading for folks doing live imaging)
MSL’s FIJI Macros
Other useful resources
Other imaging cores on UNC’s campus
Kavli NSI iDISCO Workshop 2023 resource list
Online resources
- iBiology lectures on microscopy
- Molecular expressions – exploring the world of optics and microscopy
- Olympus microscopy resource center
- Nikon MicroscopyU
- Zeiss microscopy online campus
- Imaris homeschool
Good books ($)
Great courses ($$$)
- Analytical & Quantitative Light Microscopy (Woods Hole, MA)
- Optical Microscopy & Imaging in the Biomedical Sciences (Woods Hole, MA)
- Quantitative Imaging: From Acquisition to Analysis (Cold Spring Harbor, NY)
- Quantitative Fluorescence Microscopy (Bar Harbor, ME)