Kavli NSI iDISCO Workshop 2023 resource list
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Tissue preparation
- Official iDISCO+ website (protocol, recommended antibodies, and more)
- AdipoClear:
- Tissue clearing tips and troubleshooting presentation (courtesy of Alba Vieites Pardo and Nicolas Renier)
- Recommended antibody for cFos mapping
- Lists of validated antibodies:
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Imaging
- The basic of imaging cleared tissue presentation (Pablo Ariel)
- UltraMicroscope II user guide: HTML, PDF (3D-printed holders at the bottom of the PDF download page)
- UltraMicroscope II video tutorials for usage and maintenance.
- UltraMicroscope II step-by-step instructions
- ClearMap and Tubemap suggested imaging settings
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Visualization with Imaris Viewer and analysis with Imaris
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Visualization and analysis with Clearmap
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Data management